Phenotypic characterization of an intestinal subepithelial myofibroblast cell line

John D. Valentich, Vsevolod Popov, Jamal I. Saada, Don W. Powell

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Subepithelial myofibroblasts are located at the interface between the epithelium and lamina propria in most mucosal tissues. Their biological functions are largely unknown because a long-term cell culture model for these cells has not been available. In this report, we define the phenotypic properties of a human colonic cell line (18Co) that exhibits most of the known characteristics of intestinal subepithelial myofibroblasts in situ. These characteristics include 1) a cell shape that can be reversibly interconverted between a flattened discoid and stellate morphology, 2) intracellular organelles reminiscent of myofibroblasts and smooth muscle cells in situ, 3) expression of-smooth muscle actin, 4) plasma membrane receptors for endothelins and natriuretic peptides, and 5) regulation of epithelial sensitivity to calcium-dependent secretagogues by paracrine secretion of prostaglandins. 18Co cells provide an exploitable model to begin defining the physiological and pathophysiological functions of intestinal subepithelial myofibroblasts at the molecular level.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number5 41-5
StatePublished - 1997

Fingerprint

Myofibroblasts
Muscle
Cells
Cell Line
Endothelin Receptors
Natriuretic Peptides
Cell membranes
Cell culture
Prostaglandins
Actins
Mucous Membrane
Calcium
Cell Shape
Organelles
Smooth Muscle Myocytes
Smooth Muscle
Epithelium
Cell Culture Techniques
Cell Membrane

Keywords

  • actin
  • cytoskeleton
  • endothelin
  • epithelial-mesenchymal interactions
  • mucosa
  • natriuretic peptides

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Phenotypic characterization of an intestinal subepithelial myofibroblast cell line. / Valentich, John D.; Popov, Vsevolod; Saada, Jamal I.; Powell, Don W.

In: American Journal of Physiology - Cell Physiology, Vol. 272, No. 5 41-5, 1997.

Research output: Contribution to journalArticle

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