Phenotypic characterization of an intestinal subepithelial myofibroblast cell line

John D. Valentich, Vsevold Popov, Jamal I. Saada, Don W. Powell

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Subepithelial myofibroblasts are located at the interface between the epithelium and lamina propria in most mucosal tissues. Their biological functions are largely unknown because a long-term cell culture model for these cells has not been available. In this report, we define the phenotypic properties of a human colonic cell line (18Co) that exhibits most of the known characteristics of intestinal subepithelial myofibroblasts in situ. These characteristics include 1) a cell shape that can be reversibly interconverted between a flattened discoid and stellate morphology, 2) intracellular organelles reminiscent of myofibroblasts and smooth muscle cells in situ, 3) expression of-smooth muscle actin, 4) plasma membrane receptors for endothelins and natriuretic peptides, and 5) regulation of epithelial sensitivity to calcium-dependent secretagogues by paracrine secretion of prostaglandins. 18Co cells provide an exploitable model to begin defining the physiological and pathophysiological functions of intestinal subepithelial myofibroblasts at the molecular level.

Original languageEnglish (US)
Pages (from-to)C1513-C1524
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number5 41-5
DOIs
StatePublished - 1997

Keywords

  • actin
  • cytoskeleton
  • endothelin
  • epithelial-mesenchymal interactions
  • mucosa
  • natriuretic peptides

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Fingerprint Dive into the research topics of 'Phenotypic characterization of an intestinal subepithelial myofibroblast cell line'. Together they form a unique fingerprint.

  • Cite this