Phenyl-α-tert-butyl nitrone reverses mitochondrial decay in acute Chagas' disease

Jian Jun Wen, Vandanajay Bhatia, Vsevolod Popov, Nisha Garg

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In this study, we investigated the mechanism(s) of mitochondrial functional decline in acute Chagas' disease. Our data show a substantial decline in respiratory complex activities (39 to 58%) and ATP (38%) content in Trypanosoma cruzi-infected murine hearts compared with normal controls. These metabolic alterations were associated with an approximately fivefold increase in mitochondrial reactive oxygen species production rate, substantial oxidative insult of mitochondrial membranes and respiratory complex subunits, and >60% inhibition of mtDNA-encoded transcripts for respiratory complex subunits in infected myocardium. The antioxidant phenyl-α-tert-butyl nitrone (PBN) arrested the oxidative damage-mediated loss in mitochondrial membrane integrity, preserved redox potential-coupled mitochondrial gene expression, and improved respiratory complex activities (47 to 95% increase) and cardiac ATP level (>40% increase) in infected myocardium. Importantly, PBN resulted twofold decline in mitochondrial reactive oxygen species production rate in infected myocardium. Taken together, our data demonstrate the pathological significance of oxidative stress in metabolic decay and energy homeostasis in acute chagasic myocarditis and further suggest that oxidative injuries affecting mitochondrial integrity-dependent expression and activity of the respiratory complexes initiate a feedback cycle of electron transport chain inefficiency, increased reactive oxygen species production, and energy homeostasis in acute chagasic hearts. PBN and other mitochondria-targeted antioxidants may be useful in altering mitochondrial decay and oxidative pathology in Chagas' disease.

Original languageEnglish (US)
Pages (from-to)1953-1964
Number of pages12
JournalAmerican Journal of Pathology
Volume169
Issue number6
DOIs
StatePublished - Dec 2006

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Chagas Disease
Acute Disease
Reactive Oxygen Species
Myocardium
Mitochondrial Membranes
Homeostasis
Antioxidants
Adenosine Triphosphate
Mitochondrial Genes
Trypanosoma cruzi
Myocarditis
Electron Transport
Mitochondrial DNA
Oxidation-Reduction
Mitochondria
Oxidative Stress
Pathology
Gene Expression
Wounds and Injuries
phenyl-N-tert-butylnitrone

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Phenyl-α-tert-butyl nitrone reverses mitochondrial decay in acute Chagas' disease. / Wen, Jian Jun; Bhatia, Vandanajay; Popov, Vsevolod; Garg, Nisha.

In: American Journal of Pathology, Vol. 169, No. 6, 12.2006, p. 1953-1964.

Research output: Contribution to journalArticle

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