Phenyl N-t-butylnitrone, a reactive oxygen species scavenger, reduces zymosan-induced visceral pain in rats

Jigong Wang, Victoria Cochran, Salahadin Abdi, Jin Chung, Kyungsoon Chung, Hee Kee Kim

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To examine a possible involvement of reactive oxygen species (ROS) in visceral pain, the levels of ROS in the colon and the effect of a ROS scavenger phenyl N-t-butylnitrone (PBN) on pain were examined in zymosan-induced colitis rats. Zymosan was instilled into the colon of adult rats. The electromyograms (EMGs) of abdominal muscle contractions in response to colorectal distension (CRD) were recorded as an indicator of visceral pain. After zymosan treatment, the rats showed enhanced EMG and elevated levels of H2O2 in the colon. PBN treatment (intraperitoneal, intrathecal or intracolonic) significantly reduced the enhanced EMGs induced by zymosan. The results suggest that elevated ROS in the spinal cord and the colon are involved in visceral pain.

Original languageEnglish (US)
Pages (from-to)216-219
Number of pages4
JournalNeuroscience Letters
Issue number2
StatePublished - Jul 11 2008



  • Analgesia
  • Free radical
  • Hyperalgesia
  • PBN
  • ROS
  • Sensitization

ASJC Scopus subject areas

  • Neuroscience(all)

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