Phosphate-induced efflux of adenine nucleotides from rat-heart mitochondria

evaluation of the roles of the phosphate/hydroxyl exchanger and the dicarboxylate carrier

Douglas E. Wilson, Gregory K. Asimakis

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Upon the addition of inorganic phosphate, isolated rat-heart mitochondria released endogenous adenine nucleotides. To elucidate the mechanism of this phosphate-induced efflux, we evaluated the relative roles of three inner mitochondrial membrane carriers: the adenine nucleotide translocase, the phosphate/hydroxyl exchanger, and the dicarboxylate carrier. Atractyloside (a specific inhibitor of the adenine nucleotide translocase) prevented this efflux, but did not inhibit mitochondrial swelling. Inhibitors of the phosphate/hydroxyl exchanger (200 μM n-ethylmaleimide and 10 μM mersalyl) did not inhibit phosphate-induced efflux. 200 μM mersalyl (which inhibited both the phosphate/hydroxyl exchanger and the dicarboxylate carrier) inhibited the rate of efflux approx. 65% Phenylsuccinate and 2-n-butylmalonate (inhibitors of the dicarboxylate carrier) partially inhibited phosphate-induced efflux and adenine nucleotide translocase activity. Mersalyl (200 μM) had no effect on adenine nucleotide translocase activity. Partial inhibition of the adenine nucleotide translocase by phenylsuccinate and butylmalonate could not explain the extent of inhibition of phosphate-efflux by these agents. Moreover, the rates of adenine nucleotide efflux in the presence of phenylsuccinate, butylmalonate, or mersalyl correlated well with the ability of these agents to inhibit succinate-supported respiration. We conclude that phosphate-induced efflux of adenine nucleotides from rat heart mitochondria occurs over the adenine nucleotide translocase, and that the site of action of the phosphate is not the phosphate/hydroxyl exchanger, but is likely the dicarboxylate carrier.

    Original languageEnglish (US)
    Pages (from-to)470-479
    Number of pages10
    JournalBBA - Bioenergetics
    Volume893
    Issue number3
    DOIs
    StatePublished - Oct 7 1987

    Fingerprint

    Dicarboxylic Acid Transporters
    Heart Mitochondria
    Mitochondria
    Adenine Nucleotides
    Hydroxyl Radical
    Rats
    ATP Translocases Mitochondrial ADP
    Phosphates
    Mersalyl
    Atractyloside
    Mitochondrial Swelling
    Ethylmaleimide
    Mitochondrial Membranes
    Succinic Acid

    Keywords

    • Adenine nucleotide translocase
    • Butylmalonate
    • Mitochondrion
    • Phenylsuccinate
    • Phosphate
    • Rat heart

    ASJC Scopus subject areas

    • Biophysics
    • Medicine(all)

    Cite this

    Phosphate-induced efflux of adenine nucleotides from rat-heart mitochondria : evaluation of the roles of the phosphate/hydroxyl exchanger and the dicarboxylate carrier. / Wilson, Douglas E.; Asimakis, Gregory K.

    In: BBA - Bioenergetics, Vol. 893, No. 3, 07.10.1987, p. 470-479.

    Research output: Contribution to journalArticle

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