TY - JOUR
T1 - Phosphatidyl inositol hydrolysis after CD3 binding in human peripheral blood T cells
T2 - Inhibition by prostaglandin E2
AU - Liang, Songlin
AU - Ledbetter, Jeffrey
AU - Goodwin, James S.
PY - 1989
Y1 - 1989
N2 - Hydrolysis of phosphatidyl inositol-4,5-biphosphate, leading to generation of inositol phosphates (IPs), occurs after crosslinking CD3 antigens on the surface of murine and human T-cell lines and clones, and is thought to represent a basic mechanism of signal transduction after antigen receptor binding. Previous investigators have had difficulty demonstrating this phenomenon using human peripheral blood T-cells. In this paper we demonstrate significant IP generation after anti-CD3 stimulation of human peripheral blood lymphocytes and T-cells. The amount of IP generation is less and considerably more variable than what is obtained using a T-cell line. Also, exposure to ammonium chloride in the E-rosetting procedure totally inhibits IP generation, perhaps explaining previous unsuccessful attempts. Finally, prostaglandin E2 and other agents that raise cyclic AMP inhibit IP generation by anti-CD3 antibodies in human T-cells and a T-cell line.
AB - Hydrolysis of phosphatidyl inositol-4,5-biphosphate, leading to generation of inositol phosphates (IPs), occurs after crosslinking CD3 antigens on the surface of murine and human T-cell lines and clones, and is thought to represent a basic mechanism of signal transduction after antigen receptor binding. Previous investigators have had difficulty demonstrating this phenomenon using human peripheral blood T-cells. In this paper we demonstrate significant IP generation after anti-CD3 stimulation of human peripheral blood lymphocytes and T-cells. The amount of IP generation is less and considerably more variable than what is obtained using a T-cell line. Also, exposure to ammonium chloride in the E-rosetting procedure totally inhibits IP generation, perhaps explaining previous unsuccessful attempts. Finally, prostaglandin E2 and other agents that raise cyclic AMP inhibit IP generation by anti-CD3 antibodies in human T-cells and a T-cell line.
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U2 - 10.1016/0192-0561(89)90135-5
DO - 10.1016/0192-0561(89)90135-5
M3 - Article
C2 - 2532177
AN - SCOPUS:0024445871
SN - 0192-0561
VL - 11
SP - 809
EP - 816
JO - International Journal of Immunopharmacology
JF - International Journal of Immunopharmacology
IS - 7
ER -