Abstract
The pituitary is the central endocrine gland that regulates the functions of various target organs in the human body. Because of the pivotal regulatory role of the pituitary, it is essential to define on a global scale the components of the pituitary protein machinery, including a comprehensive characterization of the post-translational modifications of the pituitary proteins. Of particular interest is the examination of the phosphorylation status of the pituitary in health and disease. Towards the goal of global profiling of pituitary protein phosphorylation, we report here the application of the in-gel IEF-LC-MS/ MS approach to the study of the pituitary phosphoproteome. The analytical strategy combined isoelectric focusing in immobilized pH gradient strips with immobilized metal ion affinity chromatography and mass spectrometry. With this method, a total of 50 phosphorylation sites were characterized in 26 proteins. Because the investigation involved primary tissue, the findings provide a direct glimpse into the phosphoprotein machinery operating within the human pituitary tissue microenvironment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 109-120 |
| Number of pages | 12 |
| Journal | Pituitary |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 2006 |
| Externally published | Yes |
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Keywords
- Human pituitary tissue
- Isoelectric focusing
- Mass spectrometry
- Phosphoproteome
ASJC Scopus subject areas
- Endocrinology
Cite this
Phosphoproteomic analysis of the human pituitary. / Beranova-Giorgianni, Sarka; Zhao, Yingxin; Desiderio, Dominic M.; Giorgianni, Francesco.
In: Pituitary, Vol. 9, No. 2, 06.2006, p. 109-120.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphoproteomic analysis of the human pituitary
AU - Beranova-Giorgianni, Sarka
AU - Zhao, Yingxin
AU - Desiderio, Dominic M.
AU - Giorgianni, Francesco
PY - 2006/6
Y1 - 2006/6
N2 - The pituitary is the central endocrine gland that regulates the functions of various target organs in the human body. Because of the pivotal regulatory role of the pituitary, it is essential to define on a global scale the components of the pituitary protein machinery, including a comprehensive characterization of the post-translational modifications of the pituitary proteins. Of particular interest is the examination of the phosphorylation status of the pituitary in health and disease. Towards the goal of global profiling of pituitary protein phosphorylation, we report here the application of the in-gel IEF-LC-MS/ MS approach to the study of the pituitary phosphoproteome. The analytical strategy combined isoelectric focusing in immobilized pH gradient strips with immobilized metal ion affinity chromatography and mass spectrometry. With this method, a total of 50 phosphorylation sites were characterized in 26 proteins. Because the investigation involved primary tissue, the findings provide a direct glimpse into the phosphoprotein machinery operating within the human pituitary tissue microenvironment.
AB - The pituitary is the central endocrine gland that regulates the functions of various target organs in the human body. Because of the pivotal regulatory role of the pituitary, it is essential to define on a global scale the components of the pituitary protein machinery, including a comprehensive characterization of the post-translational modifications of the pituitary proteins. Of particular interest is the examination of the phosphorylation status of the pituitary in health and disease. Towards the goal of global profiling of pituitary protein phosphorylation, we report here the application of the in-gel IEF-LC-MS/ MS approach to the study of the pituitary phosphoproteome. The analytical strategy combined isoelectric focusing in immobilized pH gradient strips with immobilized metal ion affinity chromatography and mass spectrometry. With this method, a total of 50 phosphorylation sites were characterized in 26 proteins. Because the investigation involved primary tissue, the findings provide a direct glimpse into the phosphoprotein machinery operating within the human pituitary tissue microenvironment.
KW - Human pituitary tissue
KW - Isoelectric focusing
KW - Mass spectrometry
KW - Phosphoproteome
UR - http://www.scopus.com/inward/record.url?scp=33749055324&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749055324&partnerID=8YFLogxK
U2 - 10.1007/s11102-006-8916-x
DO - 10.1007/s11102-006-8916-x
M3 - Article
VL - 9
SP - 109
EP - 120
JO - Pituitary
JF - Pituitary
SN - 1386-341X
IS - 2
ER -