Phosphoproteomic analysis of the human pituitary

Sarka Beranova-Giorgianni, Yingxin Zhao, Dominic M. Desiderio, Francesco Giorgianni

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The pituitary is the central endocrine gland that regulates the functions of various target organs in the human body. Because of the pivotal regulatory role of the pituitary, it is essential to define on a global scale the components of the pituitary protein machinery, including a comprehensive characterization of the post-translational modifications of the pituitary proteins. Of particular interest is the examination of the phosphorylation status of the pituitary in health and disease. Towards the goal of global profiling of pituitary protein phosphorylation, we report here the application of the in-gel IEF-LC-MS/ MS approach to the study of the pituitary phosphoproteome. The analytical strategy combined isoelectric focusing in immobilized pH gradient strips with immobilized metal ion affinity chromatography and mass spectrometry. With this method, a total of 50 phosphorylation sites were characterized in 26 proteins. Because the investigation involved primary tissue, the findings provide a direct glimpse into the phosphoprotein machinery operating within the human pituitary tissue microenvironment.

Original languageEnglish (US)
Pages (from-to)109-120
Number of pages12
JournalPituitary
Volume9
Issue number2
DOIs
StatePublished - Jun 2006
Externally publishedYes

Fingerprint

Phosphorylation
Endocrine Glands
Proteins
Proton-Motive Force
Phosphoproteins
Isoelectric Focusing
Post Translational Protein Processing
Affinity Chromatography
Human Body
Mass Spectrometry
Gels
Metals
Ions
Health

Keywords

  • Human pituitary tissue
  • Isoelectric focusing
  • Mass spectrometry
  • Phosphoproteome

ASJC Scopus subject areas

  • Endocrinology

Cite this

Beranova-Giorgianni, S., Zhao, Y., Desiderio, D. M., & Giorgianni, F. (2006). Phosphoproteomic analysis of the human pituitary. Pituitary, 9(2), 109-120. https://doi.org/10.1007/s11102-006-8916-x

Phosphoproteomic analysis of the human pituitary. / Beranova-Giorgianni, Sarka; Zhao, Yingxin; Desiderio, Dominic M.; Giorgianni, Francesco.

In: Pituitary, Vol. 9, No. 2, 06.2006, p. 109-120.

Research output: Contribution to journalArticle

Beranova-Giorgianni, S, Zhao, Y, Desiderio, DM & Giorgianni, F 2006, 'Phosphoproteomic analysis of the human pituitary', Pituitary, vol. 9, no. 2, pp. 109-120. https://doi.org/10.1007/s11102-006-8916-x
Beranova-Giorgianni S, Zhao Y, Desiderio DM, Giorgianni F. Phosphoproteomic analysis of the human pituitary. Pituitary. 2006 Jun;9(2):109-120. https://doi.org/10.1007/s11102-006-8916-x
Beranova-Giorgianni, Sarka ; Zhao, Yingxin ; Desiderio, Dominic M. ; Giorgianni, Francesco. / Phosphoproteomic analysis of the human pituitary. In: Pituitary. 2006 ; Vol. 9, No. 2. pp. 109-120.
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