Abstract
The temperature dependence to the 31P NMR spectra of poly[d(GC)]·poly[d(GC)], d(GC)4, phenylalanine tRNA (yeast) and mixtures of poly(A) + oligo(U) is presented. The 31P NMR spectra of mixtures of complementary RNA and of the poly d(GC) self-complementary DNA provide torsional information on the phosphate ester conformation in the double, triple, and "Z" helix. The increasing downfield shift with temperature for the single-strand nucleic acids provides a measure of the change in the phosphate ester conformation in the single helix to coil conversion. A separate upfield peak (20-26% of the total phosphates) is observed at lower temperatures in the oligo(U)·poly(A) mixtures which is assigned to the double helix/triple helix. Proton NMR and UV spectra confirm the presence of the multistrand forms. The 31P chemical shift for the double helix/triple helix is 0.2-0.5 ppm upfield from the chemical shift for the single helix which in turn is 1.0 ppm upfield from the chemical shift for the random coil conformation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 580-589 |
| Number of pages | 10 |
| Journal | Biochemistry |
| Volume | 21 |
| Issue number | 3 |
| State | Published - 1982 |
| Externally published | Yes |
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- Biochemistry
Cite this
Phosphorus-31 nuclear magnetic resonance of double- and triple-helical nucleic acids. Phosphorus-31 chemical shifts as a probe of phosphorus-oxygen ester bond torsional angles. / Gorenstein, David G.; Luxon, Bruce A.; Goldfield, Evelyn M.; Lai, Kofen; Vegeais, Donna.
In: Biochemistry, Vol. 21, No. 3, 1982, p. 580-589.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorus-31 nuclear magnetic resonance of double- and triple-helical nucleic acids. Phosphorus-31 chemical shifts as a probe of phosphorus-oxygen ester bond torsional angles
AU - Gorenstein, David G.
AU - Luxon, Bruce A.
AU - Goldfield, Evelyn M.
AU - Lai, Kofen
AU - Vegeais, Donna
PY - 1982
Y1 - 1982
N2 - The temperature dependence to the 31P NMR spectra of poly[d(GC)]·poly[d(GC)], d(GC)4, phenylalanine tRNA (yeast) and mixtures of poly(A) + oligo(U) is presented. The 31P NMR spectra of mixtures of complementary RNA and of the poly d(GC) self-complementary DNA provide torsional information on the phosphate ester conformation in the double, triple, and "Z" helix. The increasing downfield shift with temperature for the single-strand nucleic acids provides a measure of the change in the phosphate ester conformation in the single helix to coil conversion. A separate upfield peak (20-26% of the total phosphates) is observed at lower temperatures in the oligo(U)·poly(A) mixtures which is assigned to the double helix/triple helix. Proton NMR and UV spectra confirm the presence of the multistrand forms. The 31P chemical shift for the double helix/triple helix is 0.2-0.5 ppm upfield from the chemical shift for the single helix which in turn is 1.0 ppm upfield from the chemical shift for the random coil conformation.
AB - The temperature dependence to the 31P NMR spectra of poly[d(GC)]·poly[d(GC)], d(GC)4, phenylalanine tRNA (yeast) and mixtures of poly(A) + oligo(U) is presented. The 31P NMR spectra of mixtures of complementary RNA and of the poly d(GC) self-complementary DNA provide torsional information on the phosphate ester conformation in the double, triple, and "Z" helix. The increasing downfield shift with temperature for the single-strand nucleic acids provides a measure of the change in the phosphate ester conformation in the single helix to coil conversion. A separate upfield peak (20-26% of the total phosphates) is observed at lower temperatures in the oligo(U)·poly(A) mixtures which is assigned to the double helix/triple helix. Proton NMR and UV spectra confirm the presence of the multistrand forms. The 31P chemical shift for the double helix/triple helix is 0.2-0.5 ppm upfield from the chemical shift for the single helix which in turn is 1.0 ppm upfield from the chemical shift for the random coil conformation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020473207&partnerID=8YFLogxK
M3 - Article
C2 - 6175342
AN - SCOPUS:0020473207
VL - 21
SP - 580
EP - 589
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 3
ER -