Phosphorus-31 nuclear magnetic resonance of double- and triple-helical nucleic acids. Phosphorus-31 chemical shifts as a probe of phosphorus-oxygen ester bond torsional angles

David G. Gorenstein, Bruce A. Luxon, Evelyn M. Goldfield, Kofen Lai, Donna Vegeais

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The temperature dependence to the 31P NMR spectra of poly[d(GC)]·poly[d(GC)], d(GC)4, phenylalanine tRNA (yeast) and mixtures of poly(A) + oligo(U) is presented. The 31P NMR spectra of mixtures of complementary RNA and of the poly d(GC) self-complementary DNA provide torsional information on the phosphate ester conformation in the double, triple, and "Z" helix. The increasing downfield shift with temperature for the single-strand nucleic acids provides a measure of the change in the phosphate ester conformation in the single helix to coil conversion. A separate upfield peak (20-26% of the total phosphates) is observed at lower temperatures in the oligo(U)·poly(A) mixtures which is assigned to the double helix/triple helix. Proton NMR and UV spectra confirm the presence of the multistrand forms. The 31P chemical shift for the double helix/triple helix is 0.2-0.5 ppm upfield from the chemical shift for the single helix which in turn is 1.0 ppm upfield from the chemical shift for the random coil conformation.

Original languageEnglish (US)
Pages (from-to)580-589
Number of pages10
JournalBiochemistry
Volume21
Issue number3
StatePublished - 1982
Externally publishedYes

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Chemical shift
Phosphorus
Nucleic Acids
Conformations
Esters
Poly A
Magnetic Resonance Spectroscopy
Phosphates
Nuclear magnetic resonance
Oxygen
Temperature
Poly A-U
Complementary RNA
Transfer RNA
Phenylalanine
Yeast
Protons
Complementary DNA
Yeasts
oligo(U)

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphorus-31 nuclear magnetic resonance of double- and triple-helical nucleic acids. Phosphorus-31 chemical shifts as a probe of phosphorus-oxygen ester bond torsional angles. / Gorenstein, David G.; Luxon, Bruce A.; Goldfield, Evelyn M.; Lai, Kofen; Vegeais, Donna.

In: Biochemistry, Vol. 21, No. 3, 1982, p. 580-589.

Research output: Contribution to journalArticle

Gorenstein, David G. ; Luxon, Bruce A. ; Goldfield, Evelyn M. ; Lai, Kofen ; Vegeais, Donna. / Phosphorus-31 nuclear magnetic resonance of double- and triple-helical nucleic acids. Phosphorus-31 chemical shifts as a probe of phosphorus-oxygen ester bond torsional angles. In: Biochemistry. 1982 ; Vol. 21, No. 3. pp. 580-589.
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