Phosphorylation of Bcl-xL after spinal cord injury

Diana M. Cittelly, Olivera Nesic-Taylor, J. Regino Perez-Polo

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Spinal cord injury (SCI)-induced functional impairment results from secondary apoptosis regulated in part by SCI-induced decreases in the antiapoptotic protein Bcl-xL. We assessed the role that Bcl-x L subcellular rerouting and posttranslational phosphorylation play in Bcl-xL decreases in a contusion model of rat SCI. Immunohistochemical analysis showed the presence of Bcl-xL in neurons and oligodendrocytes, but not in astrocytes and microglia, whereas phosphorylated Bcl-xL (P-ser62-Bcl-xL) was present only in neurons. Western blot analyses showed Bcl-xL present in mitochondria, endoplasmic reticulum, nuclei, and cytosolic extracts, whereas P-ser62-Bcl-xL was restricted to organelles. During the first 24 hr after SCI, Bcl-xL levels decreased in all fractions but with a different time course, suggesting an independent regulation of Bcl-x L shuttling from the cytosol to each compartment after SCI. SCI did not affect P-ser62-Bcl-xL levels in organelles. However, P-ser62-Bcl-xL, which was not detected in the cytosolic fraction of uninjured spinal cord, appeared in the cytosol as early as 15 min postcontusion, suggesting a role for phosphorylation in SCI-induced Bcl-x L-decreases. Using an in vitro model, we observed a correlation between levels of cytosolic phosphorylated Bcl-xL and neuronal apoptosis, supporting the hypothesis that Bcl-xL phosphorylation is proapoptotic. Activated microglia/macrophages robustly expressed Bcl-x L 7 days after SCI, and a subpopulation showing nuclear condensation also expressed P-ser62-Bcl-xL. Therefore, phosphorylation of Bcl-xL may have opposite effects in injured spinal cords: 1) it may decrease levels of the antiapoptotic Bcl-xL in neurons contributing to neuronal death, and 2) it may promote apoptosis in activated microglia/macrophages, thus curtailing the inflammatory cascades associated with SCI.

Original languageEnglish (US)
Pages (from-to)1894-1911
Number of pages18
JournalJournal of Neuroscience Research
Issue number9
StatePublished - Jul 2007
Externally publishedYes


  • Apoptosis
  • Endoplasmic reticulum
  • Injury
  • Mitochondria
  • Neuronal death
  • Phosphorylation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Phosphorylation of Bcl-xL after spinal cord injury'. Together they form a unique fingerprint.

Cite this