Phosphorylation of CREB and DARPP-32 during late LTP at hippocampal to prefrontal cortex synapses in vivo

Maïté Hotte, Sébastien Thuault, Kelly Dineley, Hugh C. Hemmings, Angus C. Nairn, Thérèse M. Jay

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Specific patterns of stimulation applied in the ventral hippocampus produce long-term potentiation (LTP) of postsynaptic synapses in the prefrontal cortex in vivo. The induction of LTP is dependent on NMDA receptors and cAMP-dependant kinase (PKA) activation. Yet little is known concerning the cellular mechanisms underlying the expression of this neocortical form of LTP. In the present study, we tested whether LTP at hippocampal to prefrontal cortex synapses leads to activation of DARPP-32 and CREB as well as defined the temporal regulation of the phosphorylation states of both proteins. Our data indicate a peak in CREB and DARPP-32 phosphorylation during the late phase of prefrontal LTP (2 h posttetanus). These findings support the hypothesis that prolonged expression of hippocampal-prefrontal cortex LTP depends on a synergistic mechanism involving phosphorylation of both CREB and DARPP-32 via activation of the cAMP/PKA-dependent pathway.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalSynapse
Volume61
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Long-Term Potentiation
Prefrontal Cortex
Synapses
Phosphorylation
N-Methyl-D-Aspartate Receptors
Hippocampus
Phosphotransferases
Proteins

Keywords

  • CREB
  • DARPP-32
  • In vivo LTP
  • Prefrontal cortex

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pharmacology
  • Advanced and Specialized Nursing

Cite this

Phosphorylation of CREB and DARPP-32 during late LTP at hippocampal to prefrontal cortex synapses in vivo. / Hotte, Maïté; Thuault, Sébastien; Dineley, Kelly; Hemmings, Hugh C.; Nairn, Angus C.; Jay, Thérèse M.

In: Synapse, Vol. 61, No. 1, 01.2007, p. 24-28.

Research output: Contribution to journalArticle

Hotte, Maïté ; Thuault, Sébastien ; Dineley, Kelly ; Hemmings, Hugh C. ; Nairn, Angus C. ; Jay, Thérèse M. / Phosphorylation of CREB and DARPP-32 during late LTP at hippocampal to prefrontal cortex synapses in vivo. In: Synapse. 2007 ; Vol. 61, No. 1. pp. 24-28.
@article{81308a15e2774db1800af368fb1e3cb6,
title = "Phosphorylation of CREB and DARPP-32 during late LTP at hippocampal to prefrontal cortex synapses in vivo",
abstract = "Specific patterns of stimulation applied in the ventral hippocampus produce long-term potentiation (LTP) of postsynaptic synapses in the prefrontal cortex in vivo. The induction of LTP is dependent on NMDA receptors and cAMP-dependant kinase (PKA) activation. Yet little is known concerning the cellular mechanisms underlying the expression of this neocortical form of LTP. In the present study, we tested whether LTP at hippocampal to prefrontal cortex synapses leads to activation of DARPP-32 and CREB as well as defined the temporal regulation of the phosphorylation states of both proteins. Our data indicate a peak in CREB and DARPP-32 phosphorylation during the late phase of prefrontal LTP (2 h posttetanus). These findings support the hypothesis that prolonged expression of hippocampal-prefrontal cortex LTP depends on a synergistic mechanism involving phosphorylation of both CREB and DARPP-32 via activation of the cAMP/PKA-dependent pathway.",
keywords = "CREB, DARPP-32, In vivo LTP, Prefrontal cortex",
author = "Ma{\"i}t{\'e} Hotte and S{\'e}bastien Thuault and Kelly Dineley and Hemmings, {Hugh C.} and Nairn, {Angus C.} and Jay, {Th{\'e}r{\`e}se M.}",
year = "2007",
month = "1",
doi = "10.1002/syn.20339",
language = "English (US)",
volume = "61",
pages = "24--28",
journal = "Synapse",
issn = "0887-4476",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Phosphorylation of CREB and DARPP-32 during late LTP at hippocampal to prefrontal cortex synapses in vivo

AU - Hotte, Maïté

AU - Thuault, Sébastien

AU - Dineley, Kelly

AU - Hemmings, Hugh C.

AU - Nairn, Angus C.

AU - Jay, Thérèse M.

PY - 2007/1

Y1 - 2007/1

N2 - Specific patterns of stimulation applied in the ventral hippocampus produce long-term potentiation (LTP) of postsynaptic synapses in the prefrontal cortex in vivo. The induction of LTP is dependent on NMDA receptors and cAMP-dependant kinase (PKA) activation. Yet little is known concerning the cellular mechanisms underlying the expression of this neocortical form of LTP. In the present study, we tested whether LTP at hippocampal to prefrontal cortex synapses leads to activation of DARPP-32 and CREB as well as defined the temporal regulation of the phosphorylation states of both proteins. Our data indicate a peak in CREB and DARPP-32 phosphorylation during the late phase of prefrontal LTP (2 h posttetanus). These findings support the hypothesis that prolonged expression of hippocampal-prefrontal cortex LTP depends on a synergistic mechanism involving phosphorylation of both CREB and DARPP-32 via activation of the cAMP/PKA-dependent pathway.

AB - Specific patterns of stimulation applied in the ventral hippocampus produce long-term potentiation (LTP) of postsynaptic synapses in the prefrontal cortex in vivo. The induction of LTP is dependent on NMDA receptors and cAMP-dependant kinase (PKA) activation. Yet little is known concerning the cellular mechanisms underlying the expression of this neocortical form of LTP. In the present study, we tested whether LTP at hippocampal to prefrontal cortex synapses leads to activation of DARPP-32 and CREB as well as defined the temporal regulation of the phosphorylation states of both proteins. Our data indicate a peak in CREB and DARPP-32 phosphorylation during the late phase of prefrontal LTP (2 h posttetanus). These findings support the hypothesis that prolonged expression of hippocampal-prefrontal cortex LTP depends on a synergistic mechanism involving phosphorylation of both CREB and DARPP-32 via activation of the cAMP/PKA-dependent pathway.

KW - CREB

KW - DARPP-32

KW - In vivo LTP

KW - Prefrontal cortex

UR - http://www.scopus.com/inward/record.url?scp=33845544104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845544104&partnerID=8YFLogxK

U2 - 10.1002/syn.20339

DO - 10.1002/syn.20339

M3 - Article

C2 - 17068779

AN - SCOPUS:33845544104

VL - 61

SP - 24

EP - 28

JO - Synapse

JF - Synapse

SN - 0887-4476

IS - 1

ER -