Phosphorylation of immunity-related GTPases by a toxoplasma gondii-secreted kinase promotes macrophage survival and virulence

Sarah J. Fentress, Michael S. Behnke, Ildiko R. Dunay, Mona Mashayekhi, Leah M. Rommereim, Barbara A. Fox, David J. Bzik, Gregory A. Taylor, Benjamin E. Turk, Cheryl F. Lichti, R. Reid Townsend, Wei Qiu, Raymond Hui, Wandy L. Beatty, L. David Sibley

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Macrophages are specialized to detect and destroy intracellular microbes and yet a number of pathogens have evolved to exploit this hostile niche. Here we demonstrate that the obligate intracellular parasite Toxoplasma gondii disarms macrophage innate clearance mechanisms by secreting a serine threonine kinase called ROP18, which binds to and phosphorylates immunity-related GTPases (IRGs). Substrate profiling of ROP18 revealed a preference for a conserved motif within switch region I of the GTPase domain, a modification predicted to disrupt IRG function. Consistent with this, expression of ROP18 was both necessary and sufficient to block recruitment of Irgb6, which was in turn required for parasite destruction. ROP18 phosphorylation of IRGs prevented clearance within inflammatory monocytes and IFN-γ-activated macrophages, conferring parasite survival in vivo and promoting virulence. IRGs are implicated in clearance of a variety of intracellular pathogens, suggesting that other virulence factors may similarly thwart this innate cellular defense mechanism.

Original languageEnglish (US)
Pages (from-to)484-495
Number of pages12
JournalCell Host and Microbe
Volume8
Issue number6
DOIs
StatePublished - Dec 16 2010
Externally publishedYes

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GTP Phosphohydrolases
Toxoplasma
Virulence
Immunity
Phosphotransferases
Macrophages
Phosphorylation
Parasites
Protein-Serine-Threonine Kinases
Virulence Factors
Monocytes

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

Fentress, S. J., Behnke, M. S., Dunay, I. R., Mashayekhi, M., Rommereim, L. M., Fox, B. A., ... Sibley, L. D. (2010). Phosphorylation of immunity-related GTPases by a toxoplasma gondii-secreted kinase promotes macrophage survival and virulence. Cell Host and Microbe, 8(6), 484-495. https://doi.org/10.1016/j.chom.2010.11.005

Phosphorylation of immunity-related GTPases by a toxoplasma gondii-secreted kinase promotes macrophage survival and virulence. / Fentress, Sarah J.; Behnke, Michael S.; Dunay, Ildiko R.; Mashayekhi, Mona; Rommereim, Leah M.; Fox, Barbara A.; Bzik, David J.; Taylor, Gregory A.; Turk, Benjamin E.; Lichti, Cheryl F.; Townsend, R. Reid; Qiu, Wei; Hui, Raymond; Beatty, Wandy L.; Sibley, L. David.

In: Cell Host and Microbe, Vol. 8, No. 6, 16.12.2010, p. 484-495.

Research output: Contribution to journalArticle

Fentress, SJ, Behnke, MS, Dunay, IR, Mashayekhi, M, Rommereim, LM, Fox, BA, Bzik, DJ, Taylor, GA, Turk, BE, Lichti, CF, Townsend, RR, Qiu, W, Hui, R, Beatty, WL & Sibley, LD 2010, 'Phosphorylation of immunity-related GTPases by a toxoplasma gondii-secreted kinase promotes macrophage survival and virulence', Cell Host and Microbe, vol. 8, no. 6, pp. 484-495. https://doi.org/10.1016/j.chom.2010.11.005
Fentress, Sarah J. ; Behnke, Michael S. ; Dunay, Ildiko R. ; Mashayekhi, Mona ; Rommereim, Leah M. ; Fox, Barbara A. ; Bzik, David J. ; Taylor, Gregory A. ; Turk, Benjamin E. ; Lichti, Cheryl F. ; Townsend, R. Reid ; Qiu, Wei ; Hui, Raymond ; Beatty, Wandy L. ; Sibley, L. David. / Phosphorylation of immunity-related GTPases by a toxoplasma gondii-secreted kinase promotes macrophage survival and virulence. In: Cell Host and Microbe. 2010 ; Vol. 8, No. 6. pp. 484-495.
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