Phosphorylation of NMDA NR1 subunits in the myenteric plexus during TNBS induced colitis

QiQi Zhou, Robert M. Caudle, Baharak Moshiree, Donald D. Price, G. Nicholas Verne

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

N-Methyl-d-aspartic acid (NMDA) receptors are known to function in the mediation of pain and have a significant role in the development of hyperalgesia following inflammation. Serine phosphorylation regulation of NMDA receptor function occurs in a variety of conditions. No studies have demonstrated a change in phosphorylation of enteric NMDA receptors following colonic inflammation. We examined the levels of NMDA NR1 phosphorylation in trinitrobenzene sulfonic acid (TNBS) induced colitis in rats and compared it to protein translation and the development of visceral hypersensitivity. We have previously, demonstrated an increase in the C1 cassette of NR1 mRNA expression at 14, 21, and 28 days following TNBS administration. In this study, we examined the NR1 serine phosphorylation at 14 days following TNBS injection. Male Sprague-Dawley rats (200-250 g) were treated with TNBS (20 mg per rat) diluted in 50% ethanol (n = 3) and vehicle controls of 50% ethanol (n = 3). TNBS and vehicle controls were administered with a 24 gauge catheter inserted into the lumen of the rat colon. The animals were sacrificed at 14 days after induction of the colitis and their distal colon was retrieved for two-dimensional (2D) western blot analysis. Serine phosphorylation of the NR1 subunit with C1 cassette appears at 14 days after TNBS injection. In contrast, there was no NR1-C1 expression in the vehicle controls and untreated normal controls. These results suggest a role for colonic-NMDA receptor phosphorylation in the development of neuronal plasticity following colonic inflammation. Phosphorylation of NR1 may partially explain visceral hypersensitivity present during colonic inflammation.

Original languageEnglish (US)
Pages (from-to)250-255
Number of pages6
JournalNeuroscience Letters
Volume406
Issue number3
DOIs
StatePublished - Oct 9 2006

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Trinitrobenzenes
Myenteric Plexus
Sulfonic Acids
Colitis
Aspartic Acid
Phosphorylation
Serine
Inflammation
Hypersensitivity
Colon
Ethanol
Injections
Neuronal Plasticity
Hyperalgesia
Protein Biosynthesis
Sprague Dawley Rats
Catheters
Western Blotting
Pain
Messenger RNA

Keywords

  • NMDA receptor
  • Phosphorylation of NMDA NR1
  • TNBS-colitis
  • Visceral pain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Phosphorylation of NMDA NR1 subunits in the myenteric plexus during TNBS induced colitis. / Zhou, QiQi; Caudle, Robert M.; Moshiree, Baharak; Price, Donald D.; Verne, G. Nicholas.

In: Neuroscience Letters, Vol. 406, No. 3, 09.10.2006, p. 250-255.

Research output: Contribution to journalArticle

Zhou, QiQi ; Caudle, Robert M. ; Moshiree, Baharak ; Price, Donald D. ; Verne, G. Nicholas. / Phosphorylation of NMDA NR1 subunits in the myenteric plexus during TNBS induced colitis. In: Neuroscience Letters. 2006 ; Vol. 406, No. 3. pp. 250-255.
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