Phylogenetic and evolutionary relationships among yellow fever virus isolates in Africa

J. P. Mutebi, H. Wang, L. Li, J. E. Bryant, Alan Barrett

Research output: Contribution to journalArticle

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Abstract

Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa. We have examined the prM/M and a portion of the E protein for a panel of 38 wild strains of YF virus from Africa representing different countries and times of isolation. Examination of the strains revealed a more complex genetic relationship than previously reported. Overall, nucleotide substitutions varied from 0 to 25.8% and amino acid substitutions varied from 0 to 9.1%. Phylogenetic analysis using parsimony and neighbor-joining algorithms identified five distinct genotypes: central/east Africa, east Africa, Angola, west Africa I, and west Africa II. Extensive variation within genotypes was observed. Members of west African genotype II and central/east African genotype differed by 2.8% or less, while west Africa genotype I varied up to 6.8% at the nucleotide level. We speculate that the former two genotypes exist in enzootic transmission cycles, while the latter is genetically more heterogeneous due to regular human epidemics. The nucleotide sequence of the Angola genotype diverged from the others by 15.7 to 23.0% but only 0.4 to 5.6% at the amino acid level, suggesting that this genotype most likely diverged from a progenitor YF virus in east/central Africa many years ago, prior to the separation of the other east/central African strains analyzed in this study, and has evolved independently. These data demonstrate that there are multiple genotypes of YF virus in Africa and suggest independent evolution of YF virus in different areas of Africa.

Original languageEnglish (US)
Pages (from-to)6999-7008
Number of pages10
JournalJournal of Virology
Volume75
Issue number15
DOIs
StatePublished - 2001

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Yellow fever virus
Genotype
genotype
phylogeny
Eastern Africa
Western Africa
Central Africa
Angola
Nucleotides
nucleotides
amino acid substitution
Amino Acid Substitution
genetic relationships

ASJC Scopus subject areas

  • Immunology

Cite this

Phylogenetic and evolutionary relationships among yellow fever virus isolates in Africa. / Mutebi, J. P.; Wang, H.; Li, L.; Bryant, J. E.; Barrett, Alan.

In: Journal of Virology, Vol. 75, No. 15, 2001, p. 6999-7008.

Research output: Contribution to journalArticle

Mutebi, J. P. ; Wang, H. ; Li, L. ; Bryant, J. E. ; Barrett, Alan. / Phylogenetic and evolutionary relationships among yellow fever virus isolates in Africa. In: Journal of Virology. 2001 ; Vol. 75, No. 15. pp. 6999-7008.
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