Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses

Dong Ying Liu, Robert B. Tesh, Amelia P A Travassos da Rosa, Clarence J. Peters, Zhanqiu Yang, Hilda Guzman, Shu Yuan Xiao

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Viruses in the Phlebovirus genus of the family Bunyaviridae cause clinical syndromes ranging from a short, self-limiting febrile illness to fatal haemorrhagic fever. The genus currently consists of 68 antigenically distinct virus serotypes, most of which have not been genetically characterized. RT-PCR with four 'cocktail' primers was performed to amplify a region of the M segment of the genome of 24 phleboviruses included in the sandfly fever Naples, sandfly fever Sicilian and Punta Toro serocomplexes. Partial M segment sequences were successfully obtained and phylogenetic analysis was performed. The three resultant genotypic lineages were consistent with serological data. The sequence divergences were 27.6% (nucleotide) and 25.7% (amino acid) within the Sicilian serocomplex, 33.7% (nucleotide) and 34.4% (amino acid) within the Naples serocomplex and 35.6% (nucleotide) and 37.5% (amino acid) within the Punta Toro serocomplex. Overall, the diversities among viruses of Sicilian, Naples and Punta Toro serocomplexes were 48.2% and 57.6% at the nucleotide and amino acid levels, respectively. This high genetic divergence may explain the difficulties in designing a consensus primer pair for the amplification of all the phleboviruses using RT-PCR. It also suggests that infection with one genotype may not completely immunize against infection with all other genotypes in a given serocomplex. These findings have implications for potential vaccine development and may help explain clinical reports of multiple episodes of sandfly fever in the same individual.

Original languageEnglish (US)
Pages (from-to)465-473
Number of pages9
JournalJournal of General Virology
Volume84
Issue number2
DOIs
StatePublished - Feb 1 2003

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Phlebovirus
Bunyaviridae
Phlebotomus Fever
Sequence Analysis
Nucleotides
Amino Acids
Viruses
Fever
Genotype
Polymerase Chain Reaction
Infection
Vaccines
Genome

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Liu, D. Y., Tesh, R. B., Travassos da Rosa, A. P. A., Peters, C. J., Yang, Z., Guzman, H., & Xiao, S. Y. (2003). Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses. Journal of General Virology, 84(2), 465-473. https://doi.org/10.1099/vir.0.18765-0

Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses. / Liu, Dong Ying; Tesh, Robert B.; Travassos da Rosa, Amelia P A; Peters, Clarence J.; Yang, Zhanqiu; Guzman, Hilda; Xiao, Shu Yuan.

In: Journal of General Virology, Vol. 84, No. 2, 01.02.2003, p. 465-473.

Research output: Contribution to journalArticle

Liu, DY, Tesh, RB, Travassos da Rosa, APA, Peters, CJ, Yang, Z, Guzman, H & Xiao, SY 2003, 'Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses', Journal of General Virology, vol. 84, no. 2, pp. 465-473. https://doi.org/10.1099/vir.0.18765-0
Liu, Dong Ying ; Tesh, Robert B. ; Travassos da Rosa, Amelia P A ; Peters, Clarence J. ; Yang, Zhanqiu ; Guzman, Hilda ; Xiao, Shu Yuan. / Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses. In: Journal of General Virology. 2003 ; Vol. 84, No. 2. pp. 465-473.
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