Phylogenetic Relationships of Dengue-2 Viruses

Joyce A. Lewis, Gwong Jen Chang, Robert S. Lanciotti, Richard M. Kinney, Leonard W. Mayer, Dennis W. Trent

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

RNA oligonucleotide fingerprinting studies on a large number of virus isolates previously demonstrated considerable genetic variation in isolates of dengue (DEN-)-2 serotype. We report the entire envelope (E) glycoprotein gene and deduced amino acid sequences of 16 DEN-2 viruses and the phylogenetic relationships of these, plus 17 additional published DEN E gene sequences. Comparison of DEN-2 E glycoprotein gene sequences revealed base substitutions scattered throughout the entire gene with as much as 22% sequence divergence. Aligned E glycoprotein amino acid sequences revealed the viruses differed by as much as 10%. There appeared to be constraints on the overall structure of the E protein to maintain biological function. Clusters of amino acid substitutions were present in the hydrophobic membrane anchor region at the carboxyl terminal end of the protein. Maximum parsimony analysis of the E gene sequences allowed construction of a phylogram indicating evolutionary relationships of the virus isolates within the DEN-2 serotype. Five genetic subtypes were identified. Phylogenetic relationships of the DEN-2 serotype and other flaviviruses based on E protein sequences reflected traditional antigenic and serologic classifications.

Original languageEnglish (US)
Pages (from-to)216-224
Number of pages9
JournalVirology
Volume197
Issue number1
DOIs
StatePublished - Nov 1993

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Dengue Virus
Dengue
Glycoproteins
Genes
Viruses
Amino Acid Sequence
Flavivirus
Proteins
Amino Acid Substitution
Oligonucleotides
RNA
Membranes
Serogroup

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Lewis, J. A., Chang, G. J., Lanciotti, R. S., Kinney, R. M., Mayer, L. W., & Trent, D. W. (1993). Phylogenetic Relationships of Dengue-2 Viruses. Virology, 197(1), 216-224. https://doi.org/10.1006/viro.1993.1582

Phylogenetic Relationships of Dengue-2 Viruses. / Lewis, Joyce A.; Chang, Gwong Jen; Lanciotti, Robert S.; Kinney, Richard M.; Mayer, Leonard W.; Trent, Dennis W.

In: Virology, Vol. 197, No. 1, 11.1993, p. 216-224.

Research output: Contribution to journalArticle

Lewis, JA, Chang, GJ, Lanciotti, RS, Kinney, RM, Mayer, LW & Trent, DW 1993, 'Phylogenetic Relationships of Dengue-2 Viruses', Virology, vol. 197, no. 1, pp. 216-224. https://doi.org/10.1006/viro.1993.1582
Lewis JA, Chang GJ, Lanciotti RS, Kinney RM, Mayer LW, Trent DW. Phylogenetic Relationships of Dengue-2 Viruses. Virology. 1993 Nov;197(1):216-224. https://doi.org/10.1006/viro.1993.1582
Lewis, Joyce A. ; Chang, Gwong Jen ; Lanciotti, Robert S. ; Kinney, Richard M. ; Mayer, Leonard W. ; Trent, Dennis W. / Phylogenetic Relationships of Dengue-2 Viruses. In: Virology. 1993 ; Vol. 197, No. 1. pp. 216-224.
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