Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease

Muralidhar L. Hegde, Corey A. Theriot, Aditi Das, Pavana M. Hegde, Zhigang Guo, Ronald K. Gary, Tapas Hazra, Binghui Shen, Sankar Mitra

Research output: Contribution to journalArticle

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Abstract

The S phase-specific activation of NEIL1 and not of the other DNA glycosylases responsible for repairing oxidatively damaged bases in mammalian genomes and the activation of NEIL1 by proliferating cell nuclear antigen (PCNA) suggested preferential action by NEIL1 in oxidized base repair during DNA replication. Here we show that NEIL1 interacts with flap endonuclease 1 (FEN-1), an essential component of the DNA replication. FEN-1 is present in the NEIL1 immunocomplex isolated from human cell extracts, and the two proteins colocalize in the nucleus. FEN-1 stimulates the activity of NEIL1 in vitro in excising 5-hydroxyuracil from duplex, bubble, forked, and single-stranded DNA substrates by up to 5-fold. The disordered region near the C terminus of NEIL1, which is dispensable for activity, is necessary and sufficient for high affinity binding to FEN-1 (KD ≅ 0.2 μM). The interacting interface of FEN-1 is localized in its disordered C-terminal region uniquely present in mammalian orthologs. Fine structure mapping identified several Lys and Arg residues in this region that form salt bridges with Asp and Glu residues in NEIL1. NEIL1 was previously shown to initiate single nucleotide excision repair, which does not require FEN-1 or PCNA. The present study shows that NEIL1 could also participate in strand displacement repair synthesis (long patch repair (LP-BER)) mediated by FEN-1 and stimulated by PCNA. Interaction between NEIL1 and FEN-1 is essential for efficient NEIL1-initiated LP-BER. These studies strongly implicate NEIL1 in a distinct subpathway of LP-BER in replicating genomes.

Original languageEnglish (US)
Pages (from-to)27028-27037
Number of pages10
JournalJournal of Biological Chemistry
Volume283
Issue number40
DOIs
StatePublished - Oct 3 2008

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Flap Endonucleases
DNA Glycosylases
Proliferating Cell Nuclear Antigen
Repair
DNA Replication
Genes
Chemical activation
Genome
Single-Stranded DNA
DNA
Viperidae
Cell Extracts
S Phase
DNA Repair
Nucleotides
Salts
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease. / Hegde, Muralidhar L.; Theriot, Corey A.; Das, Aditi; Hegde, Pavana M.; Guo, Zhigang; Gary, Ronald K.; Hazra, Tapas; Shen, Binghui; Mitra, Sankar.

In: Journal of Biological Chemistry, Vol. 283, No. 40, 03.10.2008, p. 27028-27037.

Research output: Contribution to journalArticle

Hegde, Muralidhar L. ; Theriot, Corey A. ; Das, Aditi ; Hegde, Pavana M. ; Guo, Zhigang ; Gary, Ronald K. ; Hazra, Tapas ; Shen, Binghui ; Mitra, Sankar. / Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 40. pp. 27028-27037.
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AU - Hegde, Muralidhar L.

AU - Theriot, Corey A.

AU - Das, Aditi

AU - Hegde, Pavana M.

AU - Guo, Zhigang

AU - Gary, Ronald K.

AU - Hazra, Tapas

AU - Shen, Binghui

AU - Mitra, Sankar

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N2 - The S phase-specific activation of NEIL1 and not of the other DNA glycosylases responsible for repairing oxidatively damaged bases in mammalian genomes and the activation of NEIL1 by proliferating cell nuclear antigen (PCNA) suggested preferential action by NEIL1 in oxidized base repair during DNA replication. Here we show that NEIL1 interacts with flap endonuclease 1 (FEN-1), an essential component of the DNA replication. FEN-1 is present in the NEIL1 immunocomplex isolated from human cell extracts, and the two proteins colocalize in the nucleus. FEN-1 stimulates the activity of NEIL1 in vitro in excising 5-hydroxyuracil from duplex, bubble, forked, and single-stranded DNA substrates by up to 5-fold. The disordered region near the C terminus of NEIL1, which is dispensable for activity, is necessary and sufficient for high affinity binding to FEN-1 (KD ≅ 0.2 μM). The interacting interface of FEN-1 is localized in its disordered C-terminal region uniquely present in mammalian orthologs. Fine structure mapping identified several Lys and Arg residues in this region that form salt bridges with Asp and Glu residues in NEIL1. NEIL1 was previously shown to initiate single nucleotide excision repair, which does not require FEN-1 or PCNA. The present study shows that NEIL1 could also participate in strand displacement repair synthesis (long patch repair (LP-BER)) mediated by FEN-1 and stimulated by PCNA. Interaction between NEIL1 and FEN-1 is essential for efficient NEIL1-initiated LP-BER. These studies strongly implicate NEIL1 in a distinct subpathway of LP-BER in replicating genomes.

AB - The S phase-specific activation of NEIL1 and not of the other DNA glycosylases responsible for repairing oxidatively damaged bases in mammalian genomes and the activation of NEIL1 by proliferating cell nuclear antigen (PCNA) suggested preferential action by NEIL1 in oxidized base repair during DNA replication. Here we show that NEIL1 interacts with flap endonuclease 1 (FEN-1), an essential component of the DNA replication. FEN-1 is present in the NEIL1 immunocomplex isolated from human cell extracts, and the two proteins colocalize in the nucleus. FEN-1 stimulates the activity of NEIL1 in vitro in excising 5-hydroxyuracil from duplex, bubble, forked, and single-stranded DNA substrates by up to 5-fold. The disordered region near the C terminus of NEIL1, which is dispensable for activity, is necessary and sufficient for high affinity binding to FEN-1 (KD ≅ 0.2 μM). The interacting interface of FEN-1 is localized in its disordered C-terminal region uniquely present in mammalian orthologs. Fine structure mapping identified several Lys and Arg residues in this region that form salt bridges with Asp and Glu residues in NEIL1. NEIL1 was previously shown to initiate single nucleotide excision repair, which does not require FEN-1 or PCNA. The present study shows that NEIL1 could also participate in strand displacement repair synthesis (long patch repair (LP-BER)) mediated by FEN-1 and stimulated by PCNA. Interaction between NEIL1 and FEN-1 is essential for efficient NEIL1-initiated LP-BER. These studies strongly implicate NEIL1 in a distinct subpathway of LP-BER in replicating genomes.

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