TY - JOUR
T1 - Physical heterogeneity of bovine γ-globulins
T2 - Characterization of γM and γG globulins
AU - Murphy, Frederick A.
AU - Osebold, John W.
AU - Aalund, Ole
N1 - Funding Information:
1 This investigation was supported in part by a U.S. Public Health Service Postdoctoral Fellowship, IF2 AI 19788, from the National Institute of Allergy and Infectious Diseases.
PY - 1965/10
Y1 - 1965/10
N2 - The γG globulins of bovine serum were characterized by anion exchange chromatography, immunoelectrophoresis, zone electrophoresis, ultracentrifugation, and analysis of the products of papain digestion. Their properties were similar to those of analogous components of human serum. Fast and slow γG globulins were distinguished on the basis of differences in electrophoretic migration rates, chromatographic elution positions, and biological activities (complement fixation). Antigenic differences were detectable only in immunoelectrophoretic patterns (spur formation). Bovine γM was found to have properties quite similar to those of the analogous protein in human serum as determined by the methods of gel filtration, immunoelectrophoresis, anion exchange chromatography, ultracentrifugation, and reduction with mercaptoethanol. Bovine γA was provisionally recognized in immunoelectrophoretic analyses of serum and chromatographic fractions.
AB - The γG globulins of bovine serum were characterized by anion exchange chromatography, immunoelectrophoresis, zone electrophoresis, ultracentrifugation, and analysis of the products of papain digestion. Their properties were similar to those of analogous components of human serum. Fast and slow γG globulins were distinguished on the basis of differences in electrophoretic migration rates, chromatographic elution positions, and biological activities (complement fixation). Antigenic differences were detectable only in immunoelectrophoretic patterns (spur formation). Bovine γM was found to have properties quite similar to those of the analogous protein in human serum as determined by the methods of gel filtration, immunoelectrophoresis, anion exchange chromatography, ultracentrifugation, and reduction with mercaptoethanol. Bovine γA was provisionally recognized in immunoelectrophoretic analyses of serum and chromatographic fractions.
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U2 - 10.1016/0003-9861(65)90020-2
DO - 10.1016/0003-9861(65)90020-2
M3 - Article
C2 - 4159905
AN - SCOPUS:0013803996
SN - 0003-9861
VL - 112
SP - 126
EP - 136
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -