Physical Heterogeneity of Bovine Gamma Globulins: Gamma-1M Globulin Electrophoretic Heterogeneity

In the bovine species, gamma-1M globulin occurs as an electrophoretic continuum with most molecules of near mean mobility. No electrophoretic sub-groups of particularly high concentration, as described for 7S gamma globulins, were noted. Complement-fixing antibody activity was associated with bovine gamma-1M globulin molecules whose mean electrophoretic mobility was slower than the mean mobility of the protein as a whole. The marginal extent of this manifestation argues against anticipation of as heterogeneous a distribution of particular biological activities within the gamma-1M globulin spectrum as has been shown to be the case with 7S gamma globulins.