TY - JOUR
T1 - Physical studies of rabbit muscle phosphofructokinase
T2 - Sedimentation behavior of the enzymatic reactive form
AU - Hesterberg, Lyndal K.
AU - Lee, James C.
N1 - Funding Information:
1 This research was supported by NIH Grants NS 14269 and AM 21489, a grant-in-aid from the American Heart Association, Missouri Affiliate, Inc., and Research Corporation. * Abbreviations used: PFK, phosphofructokinase; DTT, dithiothreitol.
PY - 1979/10/15
Y1 - 1979/10/15
N2 - Reacting enzyme sedimentation experiments were carried out to identify the active form of rabbit muscle phosphofructokinase in glycylglycine buffer at pH 8.55 and 23 ± 1 °C. Results from these experiments reveal one active form with s20,w = 12.2 ± 0.3 and 12.2 ± 0.3 S in a coupled enzyme and a pH-dependent dye linked system, respectively.
AB - Reacting enzyme sedimentation experiments were carried out to identify the active form of rabbit muscle phosphofructokinase in glycylglycine buffer at pH 8.55 and 23 ± 1 °C. Results from these experiments reveal one active form with s20,w = 12.2 ± 0.3 and 12.2 ± 0.3 S in a coupled enzyme and a pH-dependent dye linked system, respectively.
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U2 - 10.1016/0003-9861(79)90273-X
DO - 10.1016/0003-9861(79)90273-X
M3 - Article
C2 - 41486
AN - SCOPUS:0018792548
SN - 0003-9861
VL - 197
SP - 500
EP - 502
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -