Physical studies of rabbit muscle phosphofructokinase: Sedimentation behavior of the enzymatic reactive form

Lyndal K. Hesterberg; James C. Lee

doi:10.1016/0003-9861(79)90273-X

Physical studies of rabbit muscle phosphofructokinase: Sedimentation behavior of the enzymatic reactive form

Lyndal K. Hesterberg
, James C. Lee

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Reacting enzyme sedimentation experiments were carried out to identify the active form of rabbit muscle phosphofructokinase in glycylglycine buffer at pH 8.55 and 23 ± 1 °C. Results from these experiments reveal one active form with s_20,w = 12.2 ± 0.3 and 12.2 ± 0.3 S in a coupled enzyme and a pH-dependent dye linked system, respectively.

Original language	English (US)
Pages (from-to)	500-502
Number of pages	3
Journal	Archives of Biochemistry and Biophysics
Volume	197
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(79)90273-X
State	Published - Oct 15 1979
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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@article{381d4fee1119420fa1322e12a3799ac9,

title = "Physical studies of rabbit muscle phosphofructokinase: Sedimentation behavior of the enzymatic reactive form",

abstract = "Reacting enzyme sedimentation experiments were carried out to identify the active form of rabbit muscle phosphofructokinase in glycylglycine buffer at pH 8.55 and 23 ± 1 °C. Results from these experiments reveal one active form with s20,w = 12.2 ± 0.3 and 12.2 ± 0.3 S in a coupled enzyme and a pH-dependent dye linked system, respectively.",

author = "Hesterberg, \{Lyndal K.\} and Lee, \{James C.\}",

note = "Funding Information: 1 This research was supported by NIH Grants NS 14269 and AM 21489, a grant-in-aid from the American Heart Association, Missouri Affiliate, Inc., and Research Corporation. * Abbreviations used: PFK, phosphofructokinase; DTT, dithiothreitol.",

year = "1979",

month = oct,

day = "15",

doi = "10.1016/0003-9861(79)90273-X",

language = "English (US)",

volume = "197",

pages = "500--502",

journal = "Archives of Biochemistry and Biophysics",

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TY - JOUR

T1 - Physical studies of rabbit muscle phosphofructokinase

T2 - Sedimentation behavior of the enzymatic reactive form

AU - Hesterberg, Lyndal K.

AU - Lee, James C.

N1 - Funding Information: 1 This research was supported by NIH Grants NS 14269 and AM 21489, a grant-in-aid from the American Heart Association, Missouri Affiliate, Inc., and Research Corporation. * Abbreviations used: PFK, phosphofructokinase; DTT, dithiothreitol.

PY - 1979/10/15

Y1 - 1979/10/15

N2 - Reacting enzyme sedimentation experiments were carried out to identify the active form of rabbit muscle phosphofructokinase in glycylglycine buffer at pH 8.55 and 23 ± 1 °C. Results from these experiments reveal one active form with s20,w = 12.2 ± 0.3 and 12.2 ± 0.3 S in a coupled enzyme and a pH-dependent dye linked system, respectively.

AB - Reacting enzyme sedimentation experiments were carried out to identify the active form of rabbit muscle phosphofructokinase in glycylglycine buffer at pH 8.55 and 23 ± 1 °C. Results from these experiments reveal one active form with s20,w = 12.2 ± 0.3 and 12.2 ± 0.3 S in a coupled enzyme and a pH-dependent dye linked system, respectively.

UR - https://www.scopus.com/pages/publications/0018792548

UR - https://www.scopus.com/pages/publications/0018792548#tab=citedBy

U2 - 10.1016/0003-9861(79)90273-X

DO - 10.1016/0003-9861(79)90273-X

M3 - Article

C2 - 41486

AN - SCOPUS:0018792548

SN - 0003-9861

VL - 197

SP - 500

EP - 502

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

IS - 2

ER -

Physical studies of rabbit muscle phosphofructokinase: Sedimentation behavior of the enzymatic reactive form

Abstract

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