Physico-chemical foundations underpinning microarray and next-generation sequencing experiments

Andrew Harrison, Hans Binder, Arnaud Buhot, Conrad J. Burden, Enrico Carlon, Cynthia Gibas, Lara J. Gamble, Avraham Halperin, Jef Hooyberghs, David P. Kreil, Rastislav Levicky, Peter A. Noble, Albrecht Ott, Bernard Pettitt, Diethard Tautz, Alexander E. Pozhitkov

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43 Scopus citations

Abstract

Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, nextgeneration sequencing (NGS). A physical understanding of the hybridization process helps to determine the accuracy of these technologies. The goal of a widespread research program is to develop reliable transformations between the raw signals reported by the technologies and individual molecular concentrations from an ensemble of nucleic acids. This research has inputs from many areas, from bioinformatics and biostatistics, to theoretical and experimental biochemistry and biophysics, to computer simulations. A group of leading researchers met in Ploen Germany in 2011 to discuss present knowledge and limitations of our physico-chemical understanding of high-throughput nucleic acid technologies. This meeting inspired us to write this summary, which provides an overview of the state-of-the-art approaches based on physicochemical foundation to modeling of the nucleic acids hybridization process on solid surfaces. In addition, practical application of current knowledge is emphasized.

Original languageEnglish (US)
Pages (from-to)2779-2796
Number of pages18
JournalNucleic Acids Research
Volume41
Issue number5
DOIs
StatePublished - Mar 2013

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ASJC Scopus subject areas

  • Genetics

Cite this

Harrison, A., Binder, H., Buhot, A., Burden, C. J., Carlon, E., Gibas, C., Gamble, L. J., Halperin, A., Hooyberghs, J., Kreil, D. P., Levicky, R., Noble, P. A., Ott, A., Pettitt, B., Tautz, D., & Pozhitkov, A. E. (2013). Physico-chemical foundations underpinning microarray and next-generation sequencing experiments. Nucleic Acids Research, 41(5), 2779-2796. https://doi.org/10.1093/nar/gks1358