Physicochemical characterization of progressive changes in the Xenopus laevis egg envelope following oviductal transport and fertilization

Mary Ann Bakos, Alexander Kurosky, Jerry L. Hedrick

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    Previous studies have shown that the Xenopus laevis egg envelope exists in three forms with differing ultrastructural, macromolecular, and sperm penetrability properties. The coelomic envelope (CE) is derived from eggs released from the ovary into the body cavity of the female, the vitelline envelope (VE) from eggs which have passed through the oviduct, and the fertilization envelope (FE) from fertilized eggs. In the present study, the physicochemical characteristics of these three envelope types were differentiated. Investigation of envelope solubility, deformability, sulfhydryl reactivity, and hydrophobic dye and ferritin binding capacity demonstrated that profound physicochemical changes occur in envelope conversions CE → VE → FE. The physical strength of the envelopes, as evidenced by deformability studies, ranked FE > CE > VE. These differences were not accountable by differences in the number of disulfide bonds, although the CE sulfhydryl groups were significantly less accessible than those in the VE or FE. All three envelope forms were hydrophilic in nature, exhibiting little ability to bind 1-anilino-8-naphthalenesulfonic acid. The CE bound greater amounts of ferritin in comparison to the VE and FE, indicating the presence of a basic domain, presumably in the 43-kDa glycoprotein, which is lost upon proteolysis to 41 kDa during the CE → VE conversion. The envelope integrity of all three forms was maintained by both noncovalent and covalent (disulfide) bonds. Measurements of the effect of pH on envelope solubilization indicated the involvement of an ionizable group with pKa of 8.0 in maintaining envelope structure.

    Original languageEnglish (US)
    Pages (from-to)609-615
    Number of pages7
    JournalBiochemistry
    Volume29
    Issue number3
    StatePublished - 1990

    Fingerprint

    Xenopus laevis
    Ferritins
    Formability
    Fertilization
    Disulfides
    Ovum
    Proteolysis
    Glycoproteins
    Coloring Agents
    Solubility
    Eggs
    Oviducts
    Zygote
    Spermatozoa
    Ovary
    8-anilino-1-naphthalenesulfonic acid

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Physicochemical characterization of progressive changes in the Xenopus laevis egg envelope following oviductal transport and fertilization. / Bakos, Mary Ann; Kurosky, Alexander; Hedrick, Jerry L.

    In: Biochemistry, Vol. 29, No. 3, 1990, p. 609-615.

    Research output: Contribution to journalArticle

    Bakos, Mary Ann ; Kurosky, Alexander ; Hedrick, Jerry L. / Physicochemical characterization of progressive changes in the Xenopus laevis egg envelope following oviductal transport and fertilization. In: Biochemistry. 1990 ; Vol. 29, No. 3. pp. 609-615.
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