Physiological increments in plasma insulin concentrations have selective and different effects on synthesis of hepatic proteins in normal humans

Pierpaolo De Feo, Elena Volpi, Paola Lucidi, Guido Cruciani, Gianpaolo Reboldi, Donatella Siepi, Elmo Mannarino, Fausto Santeusanio, Paolo Brunetti, Geremia B. Bolli

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

These studies tested the hypothesis that physiological increments in plasma insulin concentrations have selective effects on the synthesis of hepatic proteins in humans. Leucine kinetics and fractional synthetic rates of albumin, fibrinogen, antithrombin III, and apoB-100 were determined in 6 normal subjects, on two different occasions during either the infusion of saline (control study) or a euglycemic-hyperinsulinemic (0.4 mU · kg-1 · min-1 for 240 min) clamp, by a primed-constant infusion of [1-14C]Leu. The insulin infusion significantly decreased the rates of nonoxidatlve Leu disposal (1.70 ± 0.10 vs. control 2.06 ± 0.09 mol · kg-1 · min-1), increased the albumin (7.2 ± 0.4 vs. 6.2 ± 0.6%/day), decreased the fibrinogen (18 ± 1 vs. 23 ± 2%/day), and antithrombin III (28 ± 3 vs. 40 ± 4%/day) fractional synthetic rate, whereas it did not affect the total apoB-100 (49 ± 5 vs. 52 ± 6%/day) fractional synthetic rate. Thus, the insulin-induced decrement in the estimates of whole-body protein synthesis (nonoxidative Leu disposal) represents the mean result of opposite effects of hyperinsulinemia on the synthesis of proteins with different functions. The positive effect of insulin on albumin synthesis may play an important anabolic role during nutrient absorption by promoting the capture of a relevant amount of dietary essential amino acids into the protein, whereas the negative effect of insulin on fibrinogen synthesis might, at least partially, account for the increased plasma fibrinogen concentrations previously reported in poorly controlled diabetic patients.

Original languageEnglish (US)
Pages (from-to)995-1002
Number of pages8
JournalDiabetes
Volume42
Issue number7
StatePublished - Jul 1993
Externally publishedYes

Fingerprint

Fibrinogen
Insulin
Liver
Apolipoprotein B-100
Albumins
Antithrombin III
Proteins
Essential Amino Acids
Hyperinsulinism
Leucine
Food

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Physiological increments in plasma insulin concentrations have selective and different effects on synthesis of hepatic proteins in normal humans. / De Feo, Pierpaolo; Volpi, Elena; Lucidi, Paola; Cruciani, Guido; Reboldi, Gianpaolo; Siepi, Donatella; Mannarino, Elmo; Santeusanio, Fausto; Brunetti, Paolo; Bolli, Geremia B.

In: Diabetes, Vol. 42, No. 7, 07.1993, p. 995-1002.

Research output: Contribution to journalArticle

De Feo, P, Volpi, E, Lucidi, P, Cruciani, G, Reboldi, G, Siepi, D, Mannarino, E, Santeusanio, F, Brunetti, P & Bolli, GB 1993, 'Physiological increments in plasma insulin concentrations have selective and different effects on synthesis of hepatic proteins in normal humans', Diabetes, vol. 42, no. 7, pp. 995-1002.
De Feo, Pierpaolo ; Volpi, Elena ; Lucidi, Paola ; Cruciani, Guido ; Reboldi, Gianpaolo ; Siepi, Donatella ; Mannarino, Elmo ; Santeusanio, Fausto ; Brunetti, Paolo ; Bolli, Geremia B. / Physiological increments in plasma insulin concentrations have selective and different effects on synthesis of hepatic proteins in normal humans. In: Diabetes. 1993 ; Vol. 42, No. 7. pp. 995-1002.
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abstract = "These studies tested the hypothesis that physiological increments in plasma insulin concentrations have selective effects on the synthesis of hepatic proteins in humans. Leucine kinetics and fractional synthetic rates of albumin, fibrinogen, antithrombin III, and apoB-100 were determined in 6 normal subjects, on two different occasions during either the infusion of saline (control study) or a euglycemic-hyperinsulinemic (0.4 mU · kg-1 · min-1 for 240 min) clamp, by a primed-constant infusion of [1-14C]Leu. The insulin infusion significantly decreased the rates of nonoxidatlve Leu disposal (1.70 ± 0.10 vs. control 2.06 ± 0.09 mol · kg-1 · min-1), increased the albumin (7.2 ± 0.4 vs. 6.2 ± 0.6{\%}/day), decreased the fibrinogen (18 ± 1 vs. 23 ± 2{\%}/day), and antithrombin III (28 ± 3 vs. 40 ± 4{\%}/day) fractional synthetic rate, whereas it did not affect the total apoB-100 (49 ± 5 vs. 52 ± 6{\%}/day) fractional synthetic rate. Thus, the insulin-induced decrement in the estimates of whole-body protein synthesis (nonoxidative Leu disposal) represents the mean result of opposite effects of hyperinsulinemia on the synthesis of proteins with different functions. The positive effect of insulin on albumin synthesis may play an important anabolic role during nutrient absorption by promoting the capture of a relevant amount of dietary essential amino acids into the protein, whereas the negative effect of insulin on fibrinogen synthesis might, at least partially, account for the increased plasma fibrinogen concentrations previously reported in poorly controlled diabetic patients.",
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AU - De Feo, Pierpaolo

AU - Volpi, Elena

AU - Lucidi, Paola

AU - Cruciani, Guido

AU - Reboldi, Gianpaolo

AU - Siepi, Donatella

AU - Mannarino, Elmo

AU - Santeusanio, Fausto

AU - Brunetti, Paolo

AU - Bolli, Geremia B.

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N2 - These studies tested the hypothesis that physiological increments in plasma insulin concentrations have selective effects on the synthesis of hepatic proteins in humans. Leucine kinetics and fractional synthetic rates of albumin, fibrinogen, antithrombin III, and apoB-100 were determined in 6 normal subjects, on two different occasions during either the infusion of saline (control study) or a euglycemic-hyperinsulinemic (0.4 mU · kg-1 · min-1 for 240 min) clamp, by a primed-constant infusion of [1-14C]Leu. The insulin infusion significantly decreased the rates of nonoxidatlve Leu disposal (1.70 ± 0.10 vs. control 2.06 ± 0.09 mol · kg-1 · min-1), increased the albumin (7.2 ± 0.4 vs. 6.2 ± 0.6%/day), decreased the fibrinogen (18 ± 1 vs. 23 ± 2%/day), and antithrombin III (28 ± 3 vs. 40 ± 4%/day) fractional synthetic rate, whereas it did not affect the total apoB-100 (49 ± 5 vs. 52 ± 6%/day) fractional synthetic rate. Thus, the insulin-induced decrement in the estimates of whole-body protein synthesis (nonoxidative Leu disposal) represents the mean result of opposite effects of hyperinsulinemia on the synthesis of proteins with different functions. The positive effect of insulin on albumin synthesis may play an important anabolic role during nutrient absorption by promoting the capture of a relevant amount of dietary essential amino acids into the protein, whereas the negative effect of insulin on fibrinogen synthesis might, at least partially, account for the increased plasma fibrinogen concentrations previously reported in poorly controlled diabetic patients.

AB - These studies tested the hypothesis that physiological increments in plasma insulin concentrations have selective effects on the synthesis of hepatic proteins in humans. Leucine kinetics and fractional synthetic rates of albumin, fibrinogen, antithrombin III, and apoB-100 were determined in 6 normal subjects, on two different occasions during either the infusion of saline (control study) or a euglycemic-hyperinsulinemic (0.4 mU · kg-1 · min-1 for 240 min) clamp, by a primed-constant infusion of [1-14C]Leu. The insulin infusion significantly decreased the rates of nonoxidatlve Leu disposal (1.70 ± 0.10 vs. control 2.06 ± 0.09 mol · kg-1 · min-1), increased the albumin (7.2 ± 0.4 vs. 6.2 ± 0.6%/day), decreased the fibrinogen (18 ± 1 vs. 23 ± 2%/day), and antithrombin III (28 ± 3 vs. 40 ± 4%/day) fractional synthetic rate, whereas it did not affect the total apoB-100 (49 ± 5 vs. 52 ± 6%/day) fractional synthetic rate. Thus, the insulin-induced decrement in the estimates of whole-body protein synthesis (nonoxidative Leu disposal) represents the mean result of opposite effects of hyperinsulinemia on the synthesis of proteins with different functions. The positive effect of insulin on albumin synthesis may play an important anabolic role during nutrient absorption by promoting the capture of a relevant amount of dietary essential amino acids into the protein, whereas the negative effect of insulin on fibrinogen synthesis might, at least partially, account for the increased plasma fibrinogen concentrations previously reported in poorly controlled diabetic patients.

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