Phytochemical, in vitro antioxidant and in vivo safety evaluation of leaf extracts of Tragia plukenetii

Srinivasa Reddy Bonam, Sathish Kumar Manoharan, Vijayapandi Pandy, Anji Reddy Raya, Rama Rao Nadendla, Manjunathan Jagadeesan, Ankem Narendra Babu

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

To investigate the phytochemical properties, in vitro antioxidant and in vivo safety profile of leaf extracts of Tragia plukenetii (TP). Methods: TP leaves were obtained from the south part of India (Guntur District, Andhra Pradesh) and it was extracted with different solvents (Benzene Extract (BE), Chloroform Extract (CE) and methanolic extract (ME)). These TP extracts were analyzed for the in vitro antioxidant activity by DPPH reducing power, β-carotene-linoleic acid complex and iron chelation assays followed by in vivo acute oral and dermal toxicities using Swiss mice and Wistar rats respectively. Results: The present study results revealed ME exhibited an effective and powerful antioxidant activity when compared to a standard antioxidant, Butylated hydroxytoluene (BHT). ME was found to be effective in DPPH, β-carotene-linoleic acid complex and iron chelation assays respectively. In vivo acute oral toxicity study revealed that mice treated with up to 5000 mg/kg of BE, CE and ME did not show any signs of toxicity. Furthermore, similarly, acute dermal toxicity study demonstrated that BE, CE and ME did not exhibit any signs of dermal toxicity up to 1000 mg/kg in rats. Conclusion: TP extracts possess an excellent antioxidant activity with a devoid of any signs of acute oral and dermal toxicities.

Original languageEnglish (US)
Pages (from-to)338-345
Number of pages8
JournalPharmacognosy Journal
Volume11
Issue number2
DOIs
StatePublished - Apr 2019
Externally publishedYes

Keywords

  • DPPH assay
  • Dermal toxicity
  • Iron chelation
  • Oral toxicity
  • Tragia plukenetii
  • β-carotene-linoleic acid complex

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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