Piceatannol suppresses endotoxin-induced ocular inflammation in rats

Nilesh M. Kalariya, Mohammad Shoeb, Aramati B.M. Reddy, Rahul Sawhney, Kota V. Ramana

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Anti-inflammatory effect of piceatannol, a naturally occurring polyphenol and a potent free radical scavenger, on ocular inflammation is not known. We examined the anti-inflammatory role of piceatannol in ocular inflammatory response due to endotoxin-induced uveitis (EIU) in rats. EIU was induced in Lewis rats by subcutaneous injection of lipopolysaccharide (LPS; 150 ug/rat). Piceatannol (30 mg/kg body wt, i.p) was injected either 2 h prior to or 1 h post LPS induction. A significant increase in the number of infiltrating cells, total protein, and various cytokines and chemokines in AqH were observed in the EIU rat eyes as compared to control groups. However, pre- or post-treatment of piceatannol significantly blocked the LPS-induced changes. Further, piceatannol also suppressed the expression of cyclooxygenase-2 (Cox-2), inducible nitric oxide synthase (iNOS) and activation of NF-κB in the ciliary bodies as well as retina. Further, piceatannol also inhibited the expression of Cox-2, iNOS, and phosphorylation of NF-κB in primary human non-pigmented ciliary epithelial cells (HNPECs) treated with LPS. Similarly, piceatannol also diminished LPS-induced level of NO and prostaglandin E2 in HNPECs. Thus our results demonstrate an anti-inflammatory role of piceatannol in suppressing ocular inflammation induced by endotoxin in rats.

Original languageEnglish (US)
Pages (from-to)439-446
Number of pages8
JournalInternational Immunopharmacology
Volume17
Issue number2
DOIs
StatePublished - Jan 1 2013

Keywords

  • Inflammation NF-κB Rats
  • Piceatannol
  • Uveitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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    Kalariya, N. M., Shoeb, M., Reddy, A. B. M., Sawhney, R., & Ramana, K. V. (2013). Piceatannol suppresses endotoxin-induced ocular inflammation in rats. International Immunopharmacology, 17(2), 439-446. https://doi.org/10.1016/j.intimp.2013.07.007