Picomole quantitation of ammonia by flow-through fluorometry

David W. Good, Gerald G. Vurek

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A new fiber optic fluorometer has been used to measure picomole amounts of ammonia. The method is based on the enzymatic conversion of α-ketoglutaric acid and ammonium ion to glutamate with associated oxidation of NADH. The decrease in NADH fluorescence is used to quantify sample ammonia content. The method is rapid and reproducible and is capable of resolving differences as small as 0.3 pmol between samples containing 0-7 pmol.

Original languageEnglish (US)
Pages (from-to)199-202
Number of pages4
JournalAnalytical Biochemistry
Volume130
Issue number1
DOIs
StatePublished - Apr 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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