Picomole quantitation of ammonia by flow-through fluorometry

David Good, Gerald G. Vurek

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A new fiber optic fluorometer has been used to measure picomole amounts of ammonia. The method is based on the enzymatic conversion of α-ketoglutaric acid and ammonium ion to glutamate with associated oxidation of NADH. The decrease in NADH fluorescence is used to quantify sample ammonia content. The method is rapid and reproducible and is capable of resolving differences as small as 0.3 pmol between samples containing 0-7 pmol.

Original languageEnglish (US)
Pages (from-to)199-202
Number of pages4
JournalAnalytical Biochemistry
Volume130
Issue number1
DOIs
StatePublished - Apr 1 1983
Externally publishedYes

Fingerprint

Fluorometry
Ammonia
NAD
Fluorometers
Ketoglutaric Acids
Ammonium Compounds
Fiber optics
Glutamic Acid
Fluorescence
Ions
Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Picomole quantitation of ammonia by flow-through fluorometry. / Good, David; Vurek, Gerald G.

In: Analytical Biochemistry, Vol. 130, No. 1, 01.04.1983, p. 199-202.

Research output: Contribution to journalArticle

Good, David ; Vurek, Gerald G. / Picomole quantitation of ammonia by flow-through fluorometry. In: Analytical Biochemistry. 1983 ; Vol. 130, No. 1. pp. 199-202.
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