PIEZO1 is selectively expressed in small diameter mouse DRG neurons distinct from neurons strongly expressing TRPV1

Jigong Wang, Jun-Ho La, Owen Hamill

Research output: Contribution to journalArticle

Abstract

Using a high resolution in situ hybridization technique we have measured PIEZO1, PIEZO2, and TRPV1 transcripts in mouse dorsal root ganglion (DRG) neurons. Consistent with previous studies, PIEZO2 transcripts were highly expressed in DRG neurons of all sizes, including most notably the largest diameter neurons implicated in mediating touch and proprioception. In contrast, PIEZO1 transcripts were selectively expressed in smaller DRG neurons, which are implicated in mediating nociception. Moreover, the small neurons expressing PIEZO1 were mostly distinct from those neurons that strongly expressed TRPV1, one of the channels implicated in heat-nociception. Interestingly, while PIEZO1- and TRPV1- expressing neurons form essentially non-overlapping populations, PIEZO2 showed co-expression in both populations. Using an in vivo functional test for the selective expression, we found that Yoda1, a PIEZO1-specific agonist, induced a mechanical hyperalgesia that displayed a significantly prolonged time course compared with that induced by capsaicin, a TRPV1-specific agonist. Taken together, our results indicate that PIEZO1 should be considered a potential candidate in forming the long sought channel mediating mechano-nociception.

Original languageEnglish (US)
Article number178
JournalFrontiers in Molecular Neuroscience
Volume12
DOIs
StatePublished - Jul 9 2019

Fingerprint

Spinal Ganglia
Neurons
Nociception
Proprioception
Capsaicin
Hyperalgesia
Touch
Population
In Situ Hybridization
Hot Temperature

Keywords

  • Mechanically gated channel
  • Mechano-nociception
  • Pain
  • PIEZO1
  • PIEZO2
  • TRPV1
  • Yoda1

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

@article{705bcfeedbbc4046ad0b79cf27a9cbe5,
title = "PIEZO1 is selectively expressed in small diameter mouse DRG neurons distinct from neurons strongly expressing TRPV1",
abstract = "Using a high resolution in situ hybridization technique we have measured PIEZO1, PIEZO2, and TRPV1 transcripts in mouse dorsal root ganglion (DRG) neurons. Consistent with previous studies, PIEZO2 transcripts were highly expressed in DRG neurons of all sizes, including most notably the largest diameter neurons implicated in mediating touch and proprioception. In contrast, PIEZO1 transcripts were selectively expressed in smaller DRG neurons, which are implicated in mediating nociception. Moreover, the small neurons expressing PIEZO1 were mostly distinct from those neurons that strongly expressed TRPV1, one of the channels implicated in heat-nociception. Interestingly, while PIEZO1- and TRPV1- expressing neurons form essentially non-overlapping populations, PIEZO2 showed co-expression in both populations. Using an in vivo functional test for the selective expression, we found that Yoda1, a PIEZO1-specific agonist, induced a mechanical hyperalgesia that displayed a significantly prolonged time course compared with that induced by capsaicin, a TRPV1-specific agonist. Taken together, our results indicate that PIEZO1 should be considered a potential candidate in forming the long sought channel mediating mechano-nociception.",
keywords = "Mechanically gated channel, Mechano-nociception, Pain, PIEZO1, PIEZO2, TRPV1, Yoda1",
author = "Jigong Wang and Jun-Ho La and Owen Hamill",
year = "2019",
month = "7",
day = "9",
doi = "10.3389/fnmol.2019.00178",
language = "English (US)",
volume = "12",
journal = "Frontiers in Molecular Neuroscience",
issn = "1662-5099",
publisher = "Frontiers Research Foundation",

}

TY - JOUR

T1 - PIEZO1 is selectively expressed in small diameter mouse DRG neurons distinct from neurons strongly expressing TRPV1

AU - Wang, Jigong

AU - La, Jun-Ho

AU - Hamill, Owen

PY - 2019/7/9

Y1 - 2019/7/9

N2 - Using a high resolution in situ hybridization technique we have measured PIEZO1, PIEZO2, and TRPV1 transcripts in mouse dorsal root ganglion (DRG) neurons. Consistent with previous studies, PIEZO2 transcripts were highly expressed in DRG neurons of all sizes, including most notably the largest diameter neurons implicated in mediating touch and proprioception. In contrast, PIEZO1 transcripts were selectively expressed in smaller DRG neurons, which are implicated in mediating nociception. Moreover, the small neurons expressing PIEZO1 were mostly distinct from those neurons that strongly expressed TRPV1, one of the channels implicated in heat-nociception. Interestingly, while PIEZO1- and TRPV1- expressing neurons form essentially non-overlapping populations, PIEZO2 showed co-expression in both populations. Using an in vivo functional test for the selective expression, we found that Yoda1, a PIEZO1-specific agonist, induced a mechanical hyperalgesia that displayed a significantly prolonged time course compared with that induced by capsaicin, a TRPV1-specific agonist. Taken together, our results indicate that PIEZO1 should be considered a potential candidate in forming the long sought channel mediating mechano-nociception.

AB - Using a high resolution in situ hybridization technique we have measured PIEZO1, PIEZO2, and TRPV1 transcripts in mouse dorsal root ganglion (DRG) neurons. Consistent with previous studies, PIEZO2 transcripts were highly expressed in DRG neurons of all sizes, including most notably the largest diameter neurons implicated in mediating touch and proprioception. In contrast, PIEZO1 transcripts were selectively expressed in smaller DRG neurons, which are implicated in mediating nociception. Moreover, the small neurons expressing PIEZO1 were mostly distinct from those neurons that strongly expressed TRPV1, one of the channels implicated in heat-nociception. Interestingly, while PIEZO1- and TRPV1- expressing neurons form essentially non-overlapping populations, PIEZO2 showed co-expression in both populations. Using an in vivo functional test for the selective expression, we found that Yoda1, a PIEZO1-specific agonist, induced a mechanical hyperalgesia that displayed a significantly prolonged time course compared with that induced by capsaicin, a TRPV1-specific agonist. Taken together, our results indicate that PIEZO1 should be considered a potential candidate in forming the long sought channel mediating mechano-nociception.

KW - Mechanically gated channel

KW - Mechano-nociception

KW - Pain

KW - PIEZO1

KW - PIEZO2

KW - TRPV1

KW - Yoda1

UR - http://www.scopus.com/inward/record.url?scp=85069451365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85069451365&partnerID=8YFLogxK

U2 - 10.3389/fnmol.2019.00178

DO - 10.3389/fnmol.2019.00178

M3 - Article

AN - SCOPUS:85069451365

VL - 12

JO - Frontiers in Molecular Neuroscience

JF - Frontiers in Molecular Neuroscience

SN - 1662-5099

M1 - 178

ER -