Pigment epithelial-derived factor in human fetal membranes

Cecilia Stalberg, Nathalia Noda, Jossimara Polettini, Bo Jacobson, Ramkumar Menon

Research output: Contribution to journalArticle

Abstract

Objective: Our main objective was to document, pigment epithelial-derived factor (PEDF), a secreted serine protease inhibitor with anti-angiogenic, anti-inflammatory, and anti-oxidant properties, expression in human fetal membranes from preterm prelabor rupture of the membranes (pPROM) and in in vitro cultures stimulated with cigarette smoke extract (CSE) or lipopolysaccharides (LPS), two major risk factors for pPROM (behavioral and bacterial, respectively). Method: We documented PEDF mRNA expression in clinical samples of fetal membranes from patients with pPROM using quantitative RT-PCR. Also, mRNA and protein levels were documented in fetal membranes (from normal term cesarean sections [not in labor]) in an organ explant system stimulated with CSE or lipopolysaccharide (LPS). Immunohistochemistry (IHC) was used to localize PEDF in fetal membranes. Results: We report no changes in PEDF mRNA expression in pPROM compared to term births (p = .59) or after treatment with CSE or LPS. However, by adding sulforaphane the PEDF mRNA expression increased significantly p < .000032. PEDF was localized to both amnion and chorion layers, but no difference was seen in staining intensities after CSE or LPS treatment compared to control. Conclusions: PEDF, a product of fetal membrane cells, is unaltered in pPROM or after exposure to risk factors of pPROM. The antioxidant stimulating substance sulforaphane contribute to an increase in PEDF mRNA in fetal membranes.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalJournal of Maternal-Fetal and Neonatal Medicine
DOIs
StateAccepted/In press - Jun 19 2017

Fingerprint

Extraembryonic Membranes
Rupture
Smoke
Tobacco Products
Membranes
Lipopolysaccharides
Messenger RNA
Term Birth
Chorion
Serine Proteinase Inhibitors
Amnion
Oxidants
Cesarean Section
Anti-Inflammatory Agents
Antioxidants
Immunohistochemistry
Staining and Labeling
Polymerase Chain Reaction
Therapeutics
Proteins

Keywords

  • bacterial infection
  • fetal membrane
  • pPROM
  • Serpinf1
  • smoking

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Pigment epithelial-derived factor in human fetal membranes. / Stalberg, Cecilia; Noda, Nathalia; Polettini, Jossimara; Jacobson, Bo; Menon, Ramkumar.

In: Journal of Maternal-Fetal and Neonatal Medicine, 19.06.2017, p. 1-8.

Research output: Contribution to journalArticle

Stalberg, Cecilia ; Noda, Nathalia ; Polettini, Jossimara ; Jacobson, Bo ; Menon, Ramkumar. / Pigment epithelial-derived factor in human fetal membranes. In: Journal of Maternal-Fetal and Neonatal Medicine. 2017 ; pp. 1-8.
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