Pigment versus cholesterol cholelithiasis

identification and quantification by infrared spectroscopy

B. W. Trotman, T. A. Morris, H. M. Sanchez, R. D. Soloway, J. D. Ostrow

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The authors previously reported that 27% of 92 cholecystectomized patients had pigment stones (Am. J. Dig. Dis. 19, 585-590, 1974). Using standard biochemical methods, they found that cholesterol accounted for an average of 77% of the dry weight of cholesterol stones, but that unconjugated bilirubin represented a mean of only 7% of pigment stones. This quantitation of pigment stones was limited because approximately 66% of their weight was insoluble. To characterize pigment and cholesterol stone composition further, the authors used infrared spectroscopy, a technique requiring neither crystalinity nor solubilization, to quantitate pigment, carbonate, and cholesterol in gallstones. Other organic and inorganic components of stones were measured by standard methods. By infrared spectroscopy, 2 types of pigment stones were identified: carbonate containing and noncarbonate pigment stones. Carbonate pigment stones contained significantly more calcium, carbonate, and phosphate, but less pigment than noncarbonate stones. Compared to their initial report, the total measured components of all pigment stones were increased 6 fold from 10 to 62%. Cholesterol was the major component of cholesterol stones by chemical assay or infrared spectroscopy. Among 5 cholesterol stones with limited solubility, 80% of the insoluble residue was identified as cholesterol by infrared spectroscopy. This study extends the authors' knowledge of pigment stone and cholesterol stone composition by the use of quantitative infrared spectroscopy in conjunction with standard biochemical methods; furthermore, it confirms that pigment and cholesterol stones differ in composition and form by different mechanisms.

Original languageEnglish (US)
Pages (from-to)495-498
Number of pages4
JournalGastroenterology
Volume72
Issue number3
StatePublished - 1977
Externally publishedYes

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Cholelithiasis
Spectrum Analysis
Cholesterol
Carbonates
Weights and Measures
Gallstones
Bilirubin
Solubility

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Trotman, B. W., Morris, T. A., Sanchez, H. M., Soloway, R. D., & Ostrow, J. D. (1977). Pigment versus cholesterol cholelithiasis: identification and quantification by infrared spectroscopy. Gastroenterology, 72(3), 495-498.

Pigment versus cholesterol cholelithiasis : identification and quantification by infrared spectroscopy. / Trotman, B. W.; Morris, T. A.; Sanchez, H. M.; Soloway, R. D.; Ostrow, J. D.

In: Gastroenterology, Vol. 72, No. 3, 1977, p. 495-498.

Research output: Contribution to journalArticle

Trotman, BW, Morris, TA, Sanchez, HM, Soloway, RD & Ostrow, JD 1977, 'Pigment versus cholesterol cholelithiasis: identification and quantification by infrared spectroscopy', Gastroenterology, vol. 72, no. 3, pp. 495-498.
Trotman BW, Morris TA, Sanchez HM, Soloway RD, Ostrow JD. Pigment versus cholesterol cholelithiasis: identification and quantification by infrared spectroscopy. Gastroenterology. 1977;72(3):495-498.
Trotman, B. W. ; Morris, T. A. ; Sanchez, H. M. ; Soloway, R. D. ; Ostrow, J. D. / Pigment versus cholesterol cholelithiasis : identification and quantification by infrared spectroscopy. In: Gastroenterology. 1977 ; Vol. 72, No. 3. pp. 495-498.
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