@article{dcd99e342a4847bbade9cefe0ae87ff8,
title = "Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords",
abstract = "Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tissue-clearing technology, light sheet fluorescence microscopy, and machine learning-guided imaging analysis. This combination provides a precise determination of the cell number and somatic volume of any neuron population in the spinal cords.",
keywords = "Cell Biology, Microscopy, Molecular/Chemical Probes, Neuroscience",
author = "Hao Yu and Qiang Li and Alfredo Sandoval and Gibbs, {Holly C.} and Amber English and Tiffany Dunn and John Moth and Hajira Elahi and Bo Chen",
note = "Funding Information: This study was supported by grants from University of Texas Medical Branch (UTMB) startup funds (B.C.); Mission Connect, TIRR Foundation (B.C.); UT system star award (B.C.); Wings for Life Foundation Research grant (B.C.); Chan Zuckerberg Imaging Scientist Program (H.G.); Clinical and Translational Science Award NRSA (TL1) Training Core (TL1TR001440); and NIH/NCATS (A.S.J.). Light sheet fluorescence microscopy was performed at Texas A&M University's Microscopy and Imaging Center (RRID: SCR_022128). The graphical abstract and Figure 1 were created with BioRender.com. H.Y. and B.C. designed research; H.Y. Q.L. A.S.J. T.D. J.L.M. and H.G. performed experiments; H.Y. A.S.J. A.E. and B.C. analyzed data; and H.Y. wrote the paper with the input from all authors. A.E. is an employee of Bitplane. Funding Information: This study was supported by grants from University of Texas Medical Branch (UTMB) startup funds (B.C.); Mission Connect, TIRR Foundation (B.C.); UT system star award (B.C.); Wings for Life Foundation Research grant (B.C.); Chan Zuckerberg Imaging Scientist Program (H.G.); Clinical and Translational Science Award NRSA (TL1) Training Core (TL1TR001440); and NIH/NCATS (A.S.J.). Light sheet fluorescence microscopy was performed at Texas A&M University{\textquoteright}s Microscopy and Imaging Center (RRID: SCR_022128 ). The graphical abstract and Figure 1 were created with BioRender.com . Publisher Copyright: {\textcopyright} 2022",
year = "2022",
month = dec,
day = "16",
doi = "10.1016/j.xpro.2022.101759",
language = "English (US)",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}