Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords

Hao Yu, Qiang Li, Alfredo Sandoval, Holly C. Gibbs, Amber English, Tiffany Dunn, John Moth, Hajira Elahi, Bo Chen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tissue-clearing technology, light sheet fluorescence microscopy, and machine learning-guided imaging analysis. This combination provides a precise determination of the cell number and somatic volume of any neuron population in the spinal cords.

Original languageEnglish (US)
Article number101759
JournalSTAR Protocols
Volume3
Issue number4
DOIs
StatePublished - Dec 16 2022

Keywords

  • Cell Biology
  • Microscopy
  • Molecular/Chemical Probes
  • Neuroscience

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology

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