Pirital virus (Arenaviridae) infection in the syrian golden hamster, Mesocricetus auratus

A new animal model for arenaviral hemorrhagic fever

S. Y. Xiao, H. Zhang, Y. Yang, R. B. Tesh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Adult Syrian golden hamsters inoculated intraperitoneally with Pirital virus, a recently discovered member of the Tacaribe complex of New World arenaviruses, developed a progressively severe, fatal illness with many of the pathologic features observed in fatal human cases of Lassa fever and other arenaviral hemorrhagic fevers. Most of the animals became moribund by Day 5 and were dead by Day 7 after inoculation. The most consistent histopathologic changes included interstitial pneumonitis, splenic lymphoid depletion and necrosis, and multifocal hepatic necrosis without significant inflammatory cell infiltration. The liver changes ranged from single cell death by apoptosis to coagulative necrosis of clusters of hepatocytes. Immunohistochemical studies of the liver demonstrated the presence and accumulation of Pirital virus antigen within hepatocytes as well as Kupffer cells. An in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay showed progressively increasing apoptotic activity in the liver of infected hamsters. A human hepatoblastoma cell line (Hep G2/C3A) inoculated with Pirital virus also developed progressive cell destruction and accumulation of viral antigen, as demonstrated by immunofluorescence. Results of this pilot study suggest that the Pirital virus-hamster model is a very promising new small animal model for studying the pathogenesis of arenavirus infections, particularly, the mechanism of direct virus-induced hepatic injury. It may also be useful for testing antiviral agents for treatment of arenaviral hemorrhagic fevers.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume64
Issue number3-4
StatePublished - 2001

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Arenaviridae Infections
New World Arenaviruses
Mesocricetus
Virus Diseases
Fever
Animal Models
Liver
Necrosis
Cricetinae
Hepatocytes
Lassa Fever
Kupffer Cells
DNA Nucleotidylexotransferase
Viral Antigens
Interstitial Lung Diseases
Hep G2 Cells
Antiviral Agents
Fluorescent Antibody Technique
Cell Death
Apoptosis

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Pirital virus (Arenaviridae) infection in the syrian golden hamster, Mesocricetus auratus : A new animal model for arenaviral hemorrhagic fever. / Xiao, S. Y.; Zhang, H.; Yang, Y.; Tesh, R. B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 64, No. 3-4, 2001, p. 111-118.

Research output: Contribution to journalArticle

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