PKD prevents H2O2-induced apoptosis via NF-κB and p38 MAPK in RIE-1 cells

Jun Song, Jing Li, Jingbo Qiao, Sunil Jain, B. Mark Evers, Dai H. Chung

Research output: Contribution to journalArticle

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Abstract

Previously, we demonstrated that protein kinase D (PKD) plays a protective role during H2O2-induced intestinal cell death. Here, we sought to determine whether this effect is mediated by nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs). Treatment with H2O2 activated NF-κB in RIE-1 cells; H2O2 also induced the translocation of NF-κB p65 as well as phosphorylation of IκB-α. PKD1 siRNA inhibited H2O2-induced activation, translocation of NF-κB, and phosphorylation of IκB-α. We also found that overexpression of wild type PKD1 attenuated H2O2-induced phosphorylation of p38 MAPK and its upstream activator, MAPK kinase (MKK) 3/6, whereas the phosphorylation was increased by PKD1 siRNA or kinase-dead PKD1. Phosphorylation of neither extracellular signal-regulated kinases (ERK) 1/2 nor c-Jun N-terminal kinases (JNK) was altered by PKD1 plasmids or siRNA. Our findings suggest that PKD protects intestinal cells through up-regulation of NF-κB and down-regulation of p38 MAPK.

Original languageEnglish (US)
Pages (from-to)610-614
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume378
Issue number3
DOIs
StatePublished - Jan 16 2009

Fingerprint

Phosphorylation
Reactive ion etching
p38 Mitogen-Activated Protein Kinases
Apoptosis
Small Interfering RNA
MAP Kinase Kinase 6
MAP Kinase Kinase 3
Mitogen-Activated Protein Kinase 3
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase 1
Cell death
Mitogen-Activated Protein Kinases
Plasmids
Cell Death
Phosphotransferases
Up-Regulation
Down-Regulation
Chemical activation
protein kinase D

Keywords

  • NF-κB
  • Oxidative stress
  • p38 MAPK
  • Protein kinase D

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

PKD prevents H2O2-induced apoptosis via NF-κB and p38 MAPK in RIE-1 cells. / Song, Jun; Li, Jing; Qiao, Jingbo; Jain, Sunil; Mark Evers, B.; Chung, Dai H.

In: Biochemical and Biophysical Research Communications, Vol. 378, No. 3, 16.01.2009, p. 610-614.

Research output: Contribution to journalArticle

Song, Jun ; Li, Jing ; Qiao, Jingbo ; Jain, Sunil ; Mark Evers, B. ; Chung, Dai H. / PKD prevents H2O2-induced apoptosis via NF-κB and p38 MAPK in RIE-1 cells. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 378, No. 3. pp. 610-614.
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