Placental membrane aging and HMGB1 signaling associated with human parturition

Ramkumar Menon, Faranak Behnia, Jossimara Polettini, George Saade, Judith Campisi, Michael Velarde

    Research output: Contribution to journalArticle

    47 Citations (Scopus)

    Abstract

    Aging is associated with the onset of several diseases in various organ systems; however, different tissues may age differently, rendering some of them dysfunctional sooner than others. Placental membranes (fetal amniochorionic membranes) protect the fetus throughout pregnancy, but their longevity is limited to the duration of pregnancy. The ageassociated dysfunction of these membranes is postulated to trigger parturition. Here, we investigated whether cellular senescence-the loss of cell division potential as a consequence of stress-is involved in placental membrane function at term. We show telomere reduction, p38 MAPK activation, increase in p21 expression, loss of lamin B1 loss, increase in SA-β-galactosidase, and senescence-associated secretory phenotype (SASP) gene expression in placental membranes after labor and delivery (term labor [TL]) compared to membranes prior to labor at term (term, not-in-labor [TNIL]). Exposing TNIL placental membranes to cigarette smoke extract, an oxidative stress inducer, also induced markers of cellular senescence similar to those in TL placental membranes. Bioinformatics analysis of differentially expressed SASP genes revealed HMGB1 signaling among the top pathways involved in labor. Further, we show that recombinant HMGB1 upregulates the expression of genes associated with parturition in myometrial cells. These data suggest that the natural physiologic aging of placental tissues is associated with cellular senescence and human parturition.

    Original languageEnglish (US)
    Pages (from-to)216-230
    Number of pages15
    JournalAging
    Volume8
    Issue number2
    StatePublished - 2016

    Fingerprint

    HMGB1 Protein
    Parturition
    Membranes
    Cell Aging
    Galactosidases
    Phenotype
    Gene Expression
    Extraembryonic Membranes
    Pregnancy
    Telomere
    p38 Mitogen-Activated Protein Kinases
    Computational Biology
    Smoke
    Tobacco Products
    Cell Division
    Oxidative Stress
    Fetus
    Up-Regulation

    Keywords

    • Amnion
    • Chorion
    • DAMPs
    • Fetal membranes
    • Inflammation
    • MAPK
    • Pregnancy
    • Preterm birth
    • SASP

    ASJC Scopus subject areas

    • Aging
    • Cell Biology

    Cite this

    Menon, R., Behnia, F., Polettini, J., Saade, G., Campisi, J., & Velarde, M. (2016). Placental membrane aging and HMGB1 signaling associated with human parturition. Aging, 8(2), 216-230.

    Placental membrane aging and HMGB1 signaling associated with human parturition. / Menon, Ramkumar; Behnia, Faranak; Polettini, Jossimara; Saade, George; Campisi, Judith; Velarde, Michael.

    In: Aging, Vol. 8, No. 2, 2016, p. 216-230.

    Research output: Contribution to journalArticle

    Menon, R, Behnia, F, Polettini, J, Saade, G, Campisi, J & Velarde, M 2016, 'Placental membrane aging and HMGB1 signaling associated with human parturition', Aging, vol. 8, no. 2, pp. 216-230.
    Menon R, Behnia F, Polettini J, Saade G, Campisi J, Velarde M. Placental membrane aging and HMGB1 signaling associated with human parturition. Aging. 2016;8(2):216-230.
    Menon, Ramkumar ; Behnia, Faranak ; Polettini, Jossimara ; Saade, George ; Campisi, Judith ; Velarde, Michael. / Placental membrane aging and HMGB1 signaling associated with human parturition. In: Aging. 2016 ; Vol. 8, No. 2. pp. 216-230.
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