Placental Release of Distinct DNA-associated Micro-particles into Maternal Circulation: Reflective of Gestation Time and Preeclampsia

A. F. Orozco, C. J. Jorgez, W. D. Ramos-Perez, E. J. Popek, Xiaoying Yu, C. A. Kozinetz, F. Z. Bischoff, D. E. Lewis

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. Methods: DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. Results: DNA-associated HLA-G+ MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.005), with highest levels found in the first and second trimesters. DNA-associated PLAP+ MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.006), with highest levels in the second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G+ MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G+ MPs from the plasma of normal pregnant women (p < 0.03). Conclusions: HLA-G+ and PLAP+ MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G+ MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.

Original languageEnglish (US)
Pages (from-to)891-897
Number of pages7
JournalPlacenta
Volume30
Issue number10
DOIs
StatePublished - Oct 2009
Externally publishedYes

Fingerprint

Pre-Eclampsia
HLA Antigens
Mothers
Pregnancy
DNA
Pregnant Women
Second Pregnancy Trimester
Trophoblasts
Third Pregnancy Trimester
First Pregnancy Trimester
placental alkaline phosphatase

Keywords

  • Apoptotic micro-particles
  • DNA-associated micro-particles
  • Extravillous cytotrophoblast
  • Fetal DNA
  • HLA-G
  • PLAP
  • Preeclampsia
  • Syncytiotrophoblast

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Placental Release of Distinct DNA-associated Micro-particles into Maternal Circulation : Reflective of Gestation Time and Preeclampsia. / Orozco, A. F.; Jorgez, C. J.; Ramos-Perez, W. D.; Popek, E. J.; Yu, Xiaoying; Kozinetz, C. A.; Bischoff, F. Z.; Lewis, D. E.

In: Placenta, Vol. 30, No. 10, 10.2009, p. 891-897.

Research output: Contribution to journalArticle

Orozco, AF, Jorgez, CJ, Ramos-Perez, WD, Popek, EJ, Yu, X, Kozinetz, CA, Bischoff, FZ & Lewis, DE 2009, 'Placental Release of Distinct DNA-associated Micro-particles into Maternal Circulation: Reflective of Gestation Time and Preeclampsia', Placenta, vol. 30, no. 10, pp. 891-897. https://doi.org/10.1016/j.placenta.2009.06.012
Orozco, A. F. ; Jorgez, C. J. ; Ramos-Perez, W. D. ; Popek, E. J. ; Yu, Xiaoying ; Kozinetz, C. A. ; Bischoff, F. Z. ; Lewis, D. E. / Placental Release of Distinct DNA-associated Micro-particles into Maternal Circulation : Reflective of Gestation Time and Preeclampsia. In: Placenta. 2009 ; Vol. 30, No. 10. pp. 891-897.
@article{ffffef08866c46e7b38bafd0d559d487,
title = "Placental Release of Distinct DNA-associated Micro-particles into Maternal Circulation: Reflective of Gestation Time and Preeclampsia",
abstract = "Background: The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. Methods: DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. Results: DNA-associated HLA-G+ MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.005), with highest levels found in the first and second trimesters. DNA-associated PLAP+ MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.006), with highest levels in the second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G+ MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G+ MPs from the plasma of normal pregnant women (p < 0.03). Conclusions: HLA-G+ and PLAP+ MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G+ MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.",
keywords = "Apoptotic micro-particles, DNA-associated micro-particles, Extravillous cytotrophoblast, Fetal DNA, HLA-G, PLAP, Preeclampsia, Syncytiotrophoblast",
author = "Orozco, {A. F.} and Jorgez, {C. J.} and Ramos-Perez, {W. D.} and Popek, {E. J.} and Xiaoying Yu and Kozinetz, {C. A.} and Bischoff, {F. Z.} and Lewis, {D. E.}",
year = "2009",
month = "10",
doi = "10.1016/j.placenta.2009.06.012",
language = "English (US)",
volume = "30",
pages = "891--897",
journal = "Placenta",
issn = "0143-4004",
publisher = "W.B. Saunders Ltd",
number = "10",

}

TY - JOUR

T1 - Placental Release of Distinct DNA-associated Micro-particles into Maternal Circulation

T2 - Reflective of Gestation Time and Preeclampsia

AU - Orozco, A. F.

AU - Jorgez, C. J.

AU - Ramos-Perez, W. D.

AU - Popek, E. J.

AU - Yu, Xiaoying

AU - Kozinetz, C. A.

AU - Bischoff, F. Z.

AU - Lewis, D. E.

PY - 2009/10

Y1 - 2009/10

N2 - Background: The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. Methods: DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. Results: DNA-associated HLA-G+ MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.005), with highest levels found in the first and second trimesters. DNA-associated PLAP+ MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.006), with highest levels in the second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G+ MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G+ MPs from the plasma of normal pregnant women (p < 0.03). Conclusions: HLA-G+ and PLAP+ MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G+ MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.

AB - Background: The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. Methods: DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. Results: DNA-associated HLA-G+ MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.005), with highest levels found in the first and second trimesters. DNA-associated PLAP+ MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.006), with highest levels in the second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G+ MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G+ MPs from the plasma of normal pregnant women (p < 0.03). Conclusions: HLA-G+ and PLAP+ MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G+ MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.

KW - Apoptotic micro-particles

KW - DNA-associated micro-particles

KW - Extravillous cytotrophoblast

KW - Fetal DNA

KW - HLA-G

KW - PLAP

KW - Preeclampsia

KW - Syncytiotrophoblast

UR - http://www.scopus.com/inward/record.url?scp=70149091456&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70149091456&partnerID=8YFLogxK

U2 - 10.1016/j.placenta.2009.06.012

DO - 10.1016/j.placenta.2009.06.012

M3 - Article

C2 - 19692120

AN - SCOPUS:70149091456

VL - 30

SP - 891

EP - 897

JO - Placenta

JF - Placenta

SN - 0143-4004

IS - 10

ER -