Placental Release of Distinct DNA-associated Micro-particles into Maternal Circulation: Reflective of Gestation Time and Preeclampsia

A. F. Orozco, C. J. Jorgez, W. D. Ramos-Perez, E. J. Popek, X. Yu, C. A. Kozinetz, F. Z. Bischoff, D. E. Lewis

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Background: The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. Methods: DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. Results: DNA-associated HLA-G+ MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.005), with highest levels found in the first and second trimesters. DNA-associated PLAP+ MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p < 0.006), with highest levels in the second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G+ MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G+ MPs from the plasma of normal pregnant women (p < 0.03). Conclusions: HLA-G+ and PLAP+ MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G+ MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.

Original languageEnglish (US)
Pages (from-to)891-897
Number of pages7
JournalPlacenta
Volume30
Issue number10
DOIs
StatePublished - Oct 2009
Externally publishedYes

Keywords

  • Apoptotic micro-particles
  • DNA-associated micro-particles
  • Extravillous cytotrophoblast
  • Fetal DNA
  • HLA-G
  • PLAP
  • Preeclampsia
  • Syncytiotrophoblast

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

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