Placental telomere shortening in stillbirth: A sign of premature senescence?

Francesca Ferrari, Fabio Facchinetti, George Saade, Ramkumar Menon

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Objective: The objective of this study is to investigate placental telomere shortening in unexplained stillbirths (SBs) as an indication of premature senescence.Methods: Placentas were collected from 42 unexplained SB (>22 weeks), 43 term and 15 preterm live births, at the Policlinico Hospital of Modena (Italy). DNA extracted from placentae was studied for telomere length by real time PCR. Standard curves were generated for telomere lengths from single copy gene amplifications using a reference DNA. The telomere length for each sample was derived based on the ratio of telomere length between the sample and single copy gene standard (T/S ratio). The mean ratio of placental telomere in term live births was 5.181 ± 3.841.Results: A twofold decrease in telomere length was seen in SBs (over all 2.455 ± 1.239; p <0.001). For early SBs (above 34 weeks), the T/S was 2.8884 ± 1.224 and for late SBs, the T/S was 2.207 ± 1.201, both lower than term live births (both p <0.01). T/S remained lower both in small for gestational age-SB (2.639 ± 1.619) and appropriate for gestational age-SB (2.653 ± 1.335) with no difference between these subgroups (p = ns). T/S was lower in SB compared with spontaneous preterm births (PTBs) (6.382 ± 5.525; p <0.01), whereas SBs telomere length were similar to those of preterm premature rupture of membranes (pPROM) (3.296 ± 3.599; p = ns).Conclusions: Substantial reduction in telomere length in SBs is indicative of placental senescence. These data provide mechanistic insights that premature aging may lead to placental dysfunction as an initiator of fetal demise in unexplained SBs.

    Original languageEnglish (US)
    Pages (from-to)1283-1288
    Number of pages6
    JournalJournal of Maternal-Fetal and Neonatal Medicine
    Volume29
    Issue number8
    DOIs
    StatePublished - Apr 17 2016

    Fingerprint

    Telomere Shortening
    Stillbirth
    Telomere
    Live Birth
    Term Birth
    Premature Birth
    Placenta
    Gestational Age
    Premature Aging
    Fetal Death
    Gene Amplification
    DNA
    Italy
    Real-Time Polymerase Chain Reaction

    Keywords

    • Aging
    • oxidative stress
    • pPROM
    • preterm birth
    • risk factors
    • unexplained stillbirth

    ASJC Scopus subject areas

    • Pediatrics, Perinatology, and Child Health
    • Obstetrics and Gynecology

    Cite this

    Placental telomere shortening in stillbirth : A sign of premature senescence? / Ferrari, Francesca; Facchinetti, Fabio; Saade, George; Menon, Ramkumar.

    In: Journal of Maternal-Fetal and Neonatal Medicine, Vol. 29, No. 8, 17.04.2016, p. 1283-1288.

    Research output: Contribution to journalArticle

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    abstract = "Objective: The objective of this study is to investigate placental telomere shortening in unexplained stillbirths (SBs) as an indication of premature senescence.Methods: Placentas were collected from 42 unexplained SB (>22 weeks), 43 term and 15 preterm live births, at the Policlinico Hospital of Modena (Italy). DNA extracted from placentae was studied for telomere length by real time PCR. Standard curves were generated for telomere lengths from single copy gene amplifications using a reference DNA. The telomere length for each sample was derived based on the ratio of telomere length between the sample and single copy gene standard (T/S ratio). The mean ratio of placental telomere in term live births was 5.181 ± 3.841.Results: A twofold decrease in telomere length was seen in SBs (over all 2.455 ± 1.239; p <0.001). For early SBs (above 34 weeks), the T/S was 2.8884 ± 1.224 and for late SBs, the T/S was 2.207 ± 1.201, both lower than term live births (both p <0.01). T/S remained lower both in small for gestational age-SB (2.639 ± 1.619) and appropriate for gestational age-SB (2.653 ± 1.335) with no difference between these subgroups (p = ns). T/S was lower in SB compared with spontaneous preterm births (PTBs) (6.382 ± 5.525; p <0.01), whereas SBs telomere length were similar to those of preterm premature rupture of membranes (pPROM) (3.296 ± 3.599; p = ns).Conclusions: Substantial reduction in telomere length in SBs is indicative of placental senescence. These data provide mechanistic insights that premature aging may lead to placental dysfunction as an initiator of fetal demise in unexplained SBs.",
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    KW - pPROM

    KW - preterm birth

    KW - risk factors

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