Placental transport and in vitro effects of Bisphenol A

Thit J. Mørck, Giuseppina Sorda, Nicoletta Bechi, Brian S. Rasmussen, Jesper B. Nielsen, Francesca Ietta, Erik Rytting, Line Mathiesen, Luana Paulesu, Lisbeth E. Knudsen

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalReproductive Toxicology
Volume30
Issue number1
DOIs
StatePublished - Aug 1 2010
Externally publishedYes

Keywords

  • Bisphenol A
  • Dermal permeation
  • Endocrine disruptor
  • Fetal exposure
  • In vitro
  • Placental transport
  • Trophoblasts

ASJC Scopus subject areas

  • Toxicology

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  • Cite this

    Mørck, T. J., Sorda, G., Bechi, N., Rasmussen, B. S., Nielsen, J. B., Ietta, F., Rytting, E., Mathiesen, L., Paulesu, L., & Knudsen, L. E. (2010). Placental transport and in vitro effects of Bisphenol A. Reproductive Toxicology, 30(1), 131-137. https://doi.org/10.1016/j.reprotox.2010.02.007