Placental transport and in vitro effects of Bisphenol A

Thit J. Mørck, Giuseppina Sorda, Nicoletta Bechi, Brian S. Rasmussen, Jesper B. Nielsen, Francesca Ietta, Erik Rytting, Line Mathiesen, Luana Paulesu, Lisbeth E. Knudsen

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalReproductive Toxicology
Volume30
Issue number1
DOIs
StatePublished - Aug 2010
Externally publishedYes

Fingerprint

Fetal Development
Placentation
Consumer products
Trophoblasts
Human Development
Cytotoxicity
bisphenol A
In Vitro Techniques
Cell culture
Caspase 3
Risk assessment
Placenta
Toxicity
Monolayers
Skin
Estrogens
Perfusion
Cells
Cell Line
Pregnancy

Keywords

  • Bisphenol A
  • Dermal permeation
  • Endocrine disruptor
  • Fetal exposure
  • In vitro
  • Placental transport
  • Trophoblasts

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Cite this

Mørck, T. J., Sorda, G., Bechi, N., Rasmussen, B. S., Nielsen, J. B., Ietta, F., ... Knudsen, L. E. (2010). Placental transport and in vitro effects of Bisphenol A. Reproductive Toxicology, 30(1), 131-137. https://doi.org/10.1016/j.reprotox.2010.02.007

Placental transport and in vitro effects of Bisphenol A. / Mørck, Thit J.; Sorda, Giuseppina; Bechi, Nicoletta; Rasmussen, Brian S.; Nielsen, Jesper B.; Ietta, Francesca; Rytting, Erik; Mathiesen, Line; Paulesu, Luana; Knudsen, Lisbeth E.

In: Reproductive Toxicology, Vol. 30, No. 1, 08.2010, p. 131-137.

Research output: Contribution to journalArticle

Mørck, TJ, Sorda, G, Bechi, N, Rasmussen, BS, Nielsen, JB, Ietta, F, Rytting, E, Mathiesen, L, Paulesu, L & Knudsen, LE 2010, 'Placental transport and in vitro effects of Bisphenol A', Reproductive Toxicology, vol. 30, no. 1, pp. 131-137. https://doi.org/10.1016/j.reprotox.2010.02.007
Mørck TJ, Sorda G, Bechi N, Rasmussen BS, Nielsen JB, Ietta F et al. Placental transport and in vitro effects of Bisphenol A. Reproductive Toxicology. 2010 Aug;30(1):131-137. https://doi.org/10.1016/j.reprotox.2010.02.007
Mørck, Thit J. ; Sorda, Giuseppina ; Bechi, Nicoletta ; Rasmussen, Brian S. ; Nielsen, Jesper B. ; Ietta, Francesca ; Rytting, Erik ; Mathiesen, Line ; Paulesu, Luana ; Knudsen, Lisbeth E. / Placental transport and in vitro effects of Bisphenol A. In: Reproductive Toxicology. 2010 ; Vol. 30, No. 1. pp. 131-137.
@article{4e5c8b3e54ef423d9c1669625db65070,
title = "Placental transport and in vitro effects of Bisphenol A",
abstract = "Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.",
keywords = "Bisphenol A, Dermal permeation, Endocrine disruptor, Fetal exposure, In vitro, Placental transport, Trophoblasts",
author = "M{\o}rck, {Thit J.} and Giuseppina Sorda and Nicoletta Bechi and Rasmussen, {Brian S.} and Nielsen, {Jesper B.} and Francesca Ietta and Erik Rytting and Line Mathiesen and Luana Paulesu and Knudsen, {Lisbeth E.}",
year = "2010",
month = "8",
doi = "10.1016/j.reprotox.2010.02.007",
language = "English (US)",
volume = "30",
pages = "131--137",
journal = "Reproductigve Toxicoloy",
issn = "0890-6238",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Placental transport and in vitro effects of Bisphenol A

AU - Mørck, Thit J.

AU - Sorda, Giuseppina

AU - Bechi, Nicoletta

AU - Rasmussen, Brian S.

AU - Nielsen, Jesper B.

AU - Ietta, Francesca

AU - Rytting, Erik

AU - Mathiesen, Line

AU - Paulesu, Luana

AU - Knudsen, Lisbeth E.

PY - 2010/8

Y1 - 2010/8

N2 - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

AB - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

KW - Bisphenol A

KW - Dermal permeation

KW - Endocrine disruptor

KW - Fetal exposure

KW - In vitro

KW - Placental transport

KW - Trophoblasts

UR - http://www.scopus.com/inward/record.url?scp=77954089848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954089848&partnerID=8YFLogxK

U2 - 10.1016/j.reprotox.2010.02.007

DO - 10.1016/j.reprotox.2010.02.007

M3 - Article

C2 - 20214975

AN - SCOPUS:77954089848

VL - 30

SP - 131

EP - 137

JO - Reproductigve Toxicoloy

JF - Reproductigve Toxicoloy

SN - 0890-6238

IS - 1

ER -