Placental Trophoblast Cell Isolation from the Term Placenta

Manuel Vidal Jr. S, Radnaa Enkhtuya, Rheanna Urrabaz-Garza, Ramkumar Menon

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Multiple cell lines have been utilized over time in studying placental biology. Still, most of them rely on choriocarcinoma cells or immortalized trophoblast cells that may not be entirely comparable with actual human placental trophoblast cells. Term placentas can be a source of primary villous trophoblasts. However, challenges remain in isolating them and maintaining them in extended culture. This manuscript describes our three-phase protocol utilizing enzymatic/mechanical digestion, modified Percoll gradient density separation, and immunopurification using magnetic beads. The resulting trophoblast culture remains viable for an extended period and highly pure after initial passaging.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages131-142
Number of pages12
DOIs
StatePublished - 2024
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2781
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell culture
  • Feto-maternal interface
  • Pregnancy
  • Term placenta
  • Trophoblast

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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