Plant expression of trans-encapsidated viral nanoparticle vaccines with animal RNA replicons

Yiyang Zhou, Alison A. McCormick, Christopher M. Kearney

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

In this protocol, we outline how to produce a live viral nanoparticle vaccine in a biosafety level 1 (BSL1) environment. An animal viral vector RNA encapsidated with tobacco mosaic virus (TMV) coat protein can be fully assembled in planta. Agrobacterium cultures containing each component are inoculated together into tobacco leaves and the self-assembled hybrid nanoparticle vaccine is harvested 4 days later and purified with a simple PEG precipitation. The viral RNA delivery vector is derived from the BSL1 insect virus, Flock House virus (FHV), and replicates in human and animal cells but does not spread systemically. A polyethylene glycol purification protocol is also provided to collect and purify these vaccines for immunological tests.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages77-86
Number of pages10
Volume1499
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1499
ISSN (Print)10643745

Fingerprint

Viral Vaccines
Replicon
Viral RNA
Nanoparticles
Vaccines
Insect Viruses
RNA
Tobacco Mosaic Virus
Agrobacterium
Immunologic Tests
Capsid Proteins
Tobacco
Viruses

Keywords

  • Agroinoculation
  • Polyethylene glycol purification
  • Trans-encapsidation
  • Viral vaccine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zhou, Y., McCormick, A. A., & Kearney, C. M. (2017). Plant expression of trans-encapsidated viral nanoparticle vaccines with animal RNA replicons. In Methods in Molecular Biology (Vol. 1499, pp. 77-86). (Methods in Molecular Biology; Vol. 1499). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6481-9_4

Plant expression of trans-encapsidated viral nanoparticle vaccines with animal RNA replicons. / Zhou, Yiyang; McCormick, Alison A.; Kearney, Christopher M.

Methods in Molecular Biology. Vol. 1499 Humana Press Inc., 2017. p. 77-86 (Methods in Molecular Biology; Vol. 1499).

Research output: Chapter in Book/Report/Conference proceedingChapter

Zhou, Y, McCormick, AA & Kearney, CM 2017, Plant expression of trans-encapsidated viral nanoparticle vaccines with animal RNA replicons. in Methods in Molecular Biology. vol. 1499, Methods in Molecular Biology, vol. 1499, Humana Press Inc., pp. 77-86. https://doi.org/10.1007/978-1-4939-6481-9_4
Zhou Y, McCormick AA, Kearney CM. Plant expression of trans-encapsidated viral nanoparticle vaccines with animal RNA replicons. In Methods in Molecular Biology. Vol. 1499. Humana Press Inc. 2017. p. 77-86. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6481-9_4
Zhou, Yiyang ; McCormick, Alison A. ; Kearney, Christopher M. / Plant expression of trans-encapsidated viral nanoparticle vaccines with animal RNA replicons. Methods in Molecular Biology. Vol. 1499 Humana Press Inc., 2017. pp. 77-86 (Methods in Molecular Biology).
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