Plant expression of trans-encapsidated viral nanoparticle vaccines with animal RNA replicons

Yiyang Zhou, Alison A. McCormick, Christopher M. Kearney

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


In this protocol, we outline how to produce a live viral nanoparticle vaccine in a biosafety level 1 (BSL1) environment. An animal viral vector RNA encapsidated with tobacco mosaic virus (TMV) coat protein can be fully assembled in planta. Agrobacterium cultures containing each component are inoculated together into tobacco leaves and the self-assembled hybrid nanoparticle vaccine is harvested 4 days later and purified with a simple PEG precipitation. The viral RNA delivery vector is derived from the BSL1 insect virus, Flock House virus (FHV), and replicates in human and animal cells but does not spread systemically. A polyethylene glycol purification protocol is also provided to collect and purify these vaccines for immunological tests.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Agroinoculation
  • Polyethylene glycol purification
  • Trans-encapsidation
  • Viral vaccine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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