Plant products as topical microbicide candidates: Assessment of in vitro and in vivo activity against herpes simplex virus type 2

Krystyn Z. Bourne, Nigel Bourne, Shirley F. Reising, Lawrence R. Stanberry

Research output: Contribution to journalArticle

120 Scopus citations

Abstract

There is considerable interest in developing topical microbicides; products to be used intravaginally by women for protection against sexually transmitted diseases. Many compounds derived from plants have been shown to have antimicrobial properties. We examined 19 such compounds in vitro by plaque reduction assay to determine their activity against a common sexually transmitted pathogen, herpes simplex virus type 2. Compounds with an ED50≤7.0 mg/ml were tested for efficacy in vivo. Four compounds, carrageenan lambda type IV, cineole, curcumin, and eugenol, provided significant protection (P<0.05) in a mouse model of intravaginal HSV-2 challenge. Eugenol, which provided the greatest protection in mice was also evaluated using the guinea pig model of genital HSV-2 infection where it also demonstrated significant protection. Based on these results, several plant-derived compounds appear to warrant further evaluation as potential microbicides. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)219-226
Number of pages8
JournalAntiviral research
Volume42
Issue number3
DOIs
StatePublished - Jul 1 1999

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Keywords

  • Carrageenan
  • Cineole
  • Curcumin
  • Eugenol
  • Genital herpes
  • Herpes simplex virus
  • N-9
  • Natural products
  • Nonoxynol-9
  • Plant products
  • Sexually transmitted diseases
  • Topical microbicides
  • Vaginal microbicides

ASJC Scopus subject areas

  • Pharmacology
  • Virology

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