Plasma arginine, citrulline, and ornithine kinetics in adults, with observations on nitric oxide synthesis

Leticia Castillo, M. Sanchez, J. Vogt, T. E. Chapman, T. C. Derojas-Walker, S. R. Tannenbaum, A. M. Ajami, V. R. Young

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The plasma fluxes of ornithine (Orn), arginine (Arg), and citrulline (Cit) and rate of conversion of labeled ornithine- to-citrulline (Q(Orn → Cit)) were estimated in six healthy adult men receiving an arginine-rich or arginine-free L-amino acid- based diet, each for 6 days. On day 7 an 8-h (3- h fast, 5-h fed) primed continuous intravenous infusion of L-[guanido- 15N,15N] arginine, L-[ureido-13C]citrulline, L-[5,5,2H2]ornithine, and L-[5,5,5-2H2]leucine was conducted. Mean citrulline fluxes (μmol · kg-1 · h-1) were 10.4-13.6 for the various conditions and/or diets and remained unchanged (P > 0.05). Arginine flux was lowered (P < 0.01) by 38% for fed state during arginine-free period. Ornithine fluxes for arginine- rich were (P < 0.01) reduced with the arginine-free diet. Rates of Q(Orn → Cit) declined by 30% (P < .05) during the fed arginine-free period. Short- term restriction in the dietary supply of arginine did not alter the rate of whole body nitric oxide synthesis. One subject showed a very high output of nitrate on arginine-free diet (6 times average for remaining subjects).

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume268
Issue number2 31-2
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Citrulline
Ornithine
Arginine
Nitric Oxide
Diet
Intravenous Infusions
Leucine
Nitrates

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Castillo, L., Sanchez, M., Vogt, J., Chapman, T. E., Derojas-Walker, T. C., Tannenbaum, S. R., ... Young, V. R. (1995). Plasma arginine, citrulline, and ornithine kinetics in adults, with observations on nitric oxide synthesis. American Journal of Physiology - Endocrinology and Metabolism, 268(2 31-2).

Plasma arginine, citrulline, and ornithine kinetics in adults, with observations on nitric oxide synthesis. / Castillo, Leticia; Sanchez, M.; Vogt, J.; Chapman, T. E.; Derojas-Walker, T. C.; Tannenbaum, S. R.; Ajami, A. M.; Young, V. R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 268, No. 2 31-2, 01.01.1995.

Research output: Contribution to journalArticle

Castillo, L, Sanchez, M, Vogt, J, Chapman, TE, Derojas-Walker, TC, Tannenbaum, SR, Ajami, AM & Young, VR 1995, 'Plasma arginine, citrulline, and ornithine kinetics in adults, with observations on nitric oxide synthesis', American Journal of Physiology - Endocrinology and Metabolism, vol. 268, no. 2 31-2.
Castillo, Leticia ; Sanchez, M. ; Vogt, J. ; Chapman, T. E. ; Derojas-Walker, T. C. ; Tannenbaum, S. R. ; Ajami, A. M. ; Young, V. R. / Plasma arginine, citrulline, and ornithine kinetics in adults, with observations on nitric oxide synthesis. In: American Journal of Physiology - Endocrinology and Metabolism. 1995 ; Vol. 268, No. 2 31-2.
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