Plasma arginine-vasopressin following experimental stroke

Effect of osmotherapy

Yi Chang, Tsung Ying Chen, Chih Hung Chen, Barbara J. Crain, Thomas J K Toung, Anish Bhardwaj

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Neurohumoral responses have been implicated in the pathogenesis of ischemia-evoked cerebral edema. In a well-characterized animal model of ischemic stroke, the present study was undertaken to 1) study the profile of plasma arginine-vasopressin (AVP), and 2) determine whether osmotherapy with mannitol and various concentrations of hypertonic saline (HS) solutions influence plasma AVP levels. Halothane-anesthetized adult male Wistar rats were subjected to 2 h of middle cerebral artery occlusion with the intraluminal filament technique. Plasma AVP levels (means ± SD) were significantly elevated at 24 h (42 ± 21 pg/ml), 48 h (50 ± 28 pg/ml), and 72 h (110 ± 47 pg/ml), and returned to baseline at 96 h (22 ± 15 pg/ml) following middle cerebral artery occlusion compared with sham-operated controls (14 ± 7 pg/ml). Plasma AVP levels at 72 h were significantly attenuated with 7.5% HS (37 ± 8 pg/ml; 360 ± 11 osmol/l) compared with 0.9% saline (73 ± 6; 292 ± 6 osmol/l), 3% HS (66 ± 8 pg/ml; 303 ± 12 osmol/l), or mannitol (74 ± 9 pg/ml; 313 ± 14 osmol/l) treatment. HS (7.5%) significantly attenuated water content in the ipsilateral and contralateral hemispheres compared with surgical shams, 0.9% saline, 3% HS, and mannitol treatments. Peak plasma AVP levels were not associated with direct histopathological injury to the anterior hypothalamus. Attenuation of brain water content with 7.5% HS treatment coincides with attenuated serum AVP levels, and we speculate that this may represent one additional mechanism by which osmotherapy attenuates edema associated with ischemic stroke.

Original languageEnglish (US)
Pages (from-to)1445-1451
Number of pages7
JournalJournal of Applied Physiology
Volume100
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

Arginine Vasopressin
Stroke
Mannitol
Middle Cerebral Artery Infarction
Hypertonic Saline Solutions
Anterior Hypothalamus
Water
Brain Edema
Halothane
Wistar Rats
Edema
Ischemia
Animal Models
Wounds and Injuries
Brain
Serum

Keywords

  • Hypertonic saline
  • Ischemic stroke
  • Mannitol

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Plasma arginine-vasopressin following experimental stroke : Effect of osmotherapy. / Chang, Yi; Chen, Tsung Ying; Chen, Chih Hung; Crain, Barbara J.; Toung, Thomas J K; Bhardwaj, Anish.

In: Journal of Applied Physiology, Vol. 100, No. 5, 05.2006, p. 1445-1451.

Research output: Contribution to journalArticle

Chang, Yi ; Chen, Tsung Ying ; Chen, Chih Hung ; Crain, Barbara J. ; Toung, Thomas J K ; Bhardwaj, Anish. / Plasma arginine-vasopressin following experimental stroke : Effect of osmotherapy. In: Journal of Applied Physiology. 2006 ; Vol. 100, No. 5. pp. 1445-1451.
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