Plasma biomarkers of oxidative stress and genetic variants in age-related macular degeneration

Milam A. Brantley, Melissa P. Osborn, Barton J. Sanders, Kasra A. Rezaei, Pengcheng Lu, Chun Li, Ginger L. Milne, Jiyang Cai, Paul Sternberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

• Purpose: To compare plasma levels of oxidative stress biomarkers in patients with age-related macular degeneration (AMD) and controls and to evaluate a potential relationship between biochemical markers of oxidative stress and AMD susceptibility genotypes. • Design: Prospective case-control study. • Methods: Plasma levels of oxidative stress biomarkers were determined in 77 AMD patients and 75 controls recruited from a clinical practice. Cysteine, cystine (CySS), glutathione, isoprostane, and isofuran were measured, and participants were genotyped for polymorphisms in the complement factor H (CFH) and age-related maculopathy susceptibility 2 (ARMS2) genes. • Results: CySS was elevated in cases compared with controls (P =.013). After adjustment for age, sex, and smoking, this association was not significant. In all participants, CySS levels were associated with the CFH polymorphism rs3753394 (P =.028) as well as an 8-allele CFH haplotype (P =.029) after correction for age, gender, and smoking. None of the other plasma markers was related to AMD status in our cohort. • Conclusions: Our investigation of the geneenvironment interaction involved in AMD revealed a relationship between a plasma biomarker of oxidative stress, CySS, and CFH genotype. These data suggest a potential association between inflammatory regulators and redox status in AMD pathogenesis.

Original languageEnglish (US)
Pages (from-to)460-467
Number of pages8
JournalAmerican Journal of Ophthalmology
Volume153
Issue number3
DOIs
StatePublished - Mar 2012
Externally publishedYes

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Macular Degeneration
Complement Factor H
Oxidative Stress
Cystine
Biomarkers
Cysteine
Smoking
Genotype
Isoprostanes
Haplotypes
Oxidation-Reduction
Glutathione
Case-Control Studies
Alleles
Genes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Brantley, M. A., Osborn, M. P., Sanders, B. J., Rezaei, K. A., Lu, P., Li, C., ... Sternberg, P. (2012). Plasma biomarkers of oxidative stress and genetic variants in age-related macular degeneration. American Journal of Ophthalmology, 153(3), 460-467. https://doi.org/10.1016/j.ajo.2011.08.033

Plasma biomarkers of oxidative stress and genetic variants in age-related macular degeneration. / Brantley, Milam A.; Osborn, Melissa P.; Sanders, Barton J.; Rezaei, Kasra A.; Lu, Pengcheng; Li, Chun; Milne, Ginger L.; Cai, Jiyang; Sternberg, Paul.

In: American Journal of Ophthalmology, Vol. 153, No. 3, 03.2012, p. 460-467.

Research output: Contribution to journalArticle

Brantley, MA, Osborn, MP, Sanders, BJ, Rezaei, KA, Lu, P, Li, C, Milne, GL, Cai, J & Sternberg, P 2012, 'Plasma biomarkers of oxidative stress and genetic variants in age-related macular degeneration', American Journal of Ophthalmology, vol. 153, no. 3, pp. 460-467. https://doi.org/10.1016/j.ajo.2011.08.033
Brantley, Milam A. ; Osborn, Melissa P. ; Sanders, Barton J. ; Rezaei, Kasra A. ; Lu, Pengcheng ; Li, Chun ; Milne, Ginger L. ; Cai, Jiyang ; Sternberg, Paul. / Plasma biomarkers of oxidative stress and genetic variants in age-related macular degeneration. In: American Journal of Ophthalmology. 2012 ; Vol. 153, No. 3. pp. 460-467.
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