Plasma L-5-oxoproline kinetics and whole blood glutathione synthesis rates in severely burned adult humans

Yong Ming Yu, Colleen M. Ryan, Zhe Wei Fei, Xiao Ming Lu, Leticia Castillo, John T. Schultz, Ronald G. Tompkins, Vernon R. Young

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Compromised glutathione homeostasis is associated with increased morbidity in various disease states. We evaluated the kinetics of L-5-oxoproline, an intermediate in the γ-glutamyl cycle of glutathione production, in fourteen severely burned adults by use of a primed, constant intravenous infusion of L-5-[1-13C] oxoproline. In nine of these patients, whole blood glutathione synthesis and plasma kinetics of glycine and leucine were also measured with [15N]glycine and L-[2H3]leucine tracers. Patients were studied under a "basal" condition that provided a low dose of glucose and total parenteral nutrition. For comparison with control subjects, whole blood glutathione synthesis was estimated in six healthy adults. Burn patients in a basal condition showed significantly higher rates of plasma oxoproline clearance and urinary D- and L-oxoproline excretion compared with fasting healthy control subjects. Whole blood glutathione concentration and absolute synthesis rate in the basal state were lower than for control subjects. Total parenteral feeding without cysteine but with generous methionine did not affect oxoproline kinetics or whole blood glutathione synthesis. The estimated rate of glycine de novo synthesis was also lower in burn patients, suggesting a possible change in glycine availability for glutathione synthesis. The roles of precursor amino acid availability, as well as alterations in metabolic capacity, in modulating whole blood glutathione production in burns now require investigation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume282
Issue number2 45-2
StatePublished - Jun 29 2002
Externally publishedYes

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Pyrrolidonecarboxylic Acid
Glutathione
Glycine
Leucine
Total Parenteral Nutrition
Parenteral Nutrition
Burns
Intravenous Infusions
Methionine
Cysteine
Fasting
Healthy Volunteers
Homeostasis
Morbidity
Amino Acids
Glucose

Keywords

  • Clearance
  • De novo synthesis
  • Flux
  • Glycine
  • Urinary excretion

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Plasma L-5-oxoproline kinetics and whole blood glutathione synthesis rates in severely burned adult humans. / Yu, Yong Ming; Ryan, Colleen M.; Fei, Zhe Wei; Lu, Xiao Ming; Castillo, Leticia; Schultz, John T.; Tompkins, Ronald G.; Young, Vernon R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 282, No. 2 45-2, 29.06.2002.

Research output: Contribution to journalArticle

Yu, Yong Ming ; Ryan, Colleen M. ; Fei, Zhe Wei ; Lu, Xiao Ming ; Castillo, Leticia ; Schultz, John T. ; Tompkins, Ronald G. ; Young, Vernon R. / Plasma L-5-oxoproline kinetics and whole blood glutathione synthesis rates in severely burned adult humans. In: American Journal of Physiology - Endocrinology and Metabolism. 2002 ; Vol. 282, No. 2 45-2.
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