Plasma levels of angiostatin and endostatin remain unchanged for the first 3 weeks after colorectal cancer surgery

H. M C Shantha Kumara, Samer T. Tohme, Xiaohong Yan, Abu Nasar, Anthony J. Senagore, Matthew F. Kalady, Neil Hyman, Ik Y. Kim, Richard L. Whelan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction Angiostatin and endostatin are endogenous inhibitors of angiogenesis with anticancer effects. After minimally invasive colorectal resection (MICR), blood levels of the proangiogenic factors vascular endothelial growth factor (VEGF) and angiopoetin 2 (Ang-2) are elevated for 2-4 weeks. Also, postoperative human plasma from weeks 2 and 3 after MICRhas been shown to stimulate endothelial cell proliferation and migration,which are critical to angiogenesis. This proangiogenic state may stimulate tumor growth early afterMICR. Surgery's impact on angiostatin and endostatin is unknown. This study's purpose is to determine perioperative plasma levels of these two proteins in colorectal cancer (CRC) patients undergoing MICR. Methods Endostatin levels were assessed in 34 CRC patients and angiostatin levels in 30 CRC patients. Blood samples were taken preoperatively and on postoperative day (POD) 1 and 3 in all patients; in a subset, samples were taken between POD 7 and 20. The late samples were bundled into 7-day blocks (POD 7-13, POD 14-20) and considered as single time points. Angiostatin and endostatin plasma levels were determined via enzyme-linked immunosorbent assay (ELISA) in duplicate. Wilcoxon signed-rank test and Student's t test were used to analyze endostatin and angiostatin data, respectively. Significance was set at P

Original languageEnglish (US)
Pages (from-to)1939-1944
Number of pages6
JournalSurgical Endoscopy and Other Interventional Techniques
Volume25
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

Fingerprint

Angiostatins
Endostatins
Colorectal Surgery
Colorectal Neoplasms
Angiogenesis Inhibitors
Nonparametric Statistics
Vascular Endothelial Growth Factor A
Cell Movement
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Students
Growth
Neoplasms
Proteins

Keywords

  • Angiogenesis
  • Angiostatin
  • Colon cancer
  • Colorectal resection
  • Endostatin
  • Surgery-related plasma alterations

ASJC Scopus subject areas

  • Surgery

Cite this

Plasma levels of angiostatin and endostatin remain unchanged for the first 3 weeks after colorectal cancer surgery. / Kumara, H. M C Shantha; Tohme, Samer T.; Yan, Xiaohong; Nasar, Abu; Senagore, Anthony J.; Kalady, Matthew F.; Hyman, Neil; Kim, Ik Y.; Whelan, Richard L.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 25, No. 6, 06.2011, p. 1939-1944.

Research output: Contribution to journalArticle

Kumara, HMCS, Tohme, ST, Yan, X, Nasar, A, Senagore, AJ, Kalady, MF, Hyman, N, Kim, IY & Whelan, RL 2011, 'Plasma levels of angiostatin and endostatin remain unchanged for the first 3 weeks after colorectal cancer surgery', Surgical Endoscopy and Other Interventional Techniques, vol. 25, no. 6, pp. 1939-1944. https://doi.org/10.1007/s00464-010-1491-2
Kumara, H. M C Shantha ; Tohme, Samer T. ; Yan, Xiaohong ; Nasar, Abu ; Senagore, Anthony J. ; Kalady, Matthew F. ; Hyman, Neil ; Kim, Ik Y. ; Whelan, Richard L. / Plasma levels of angiostatin and endostatin remain unchanged for the first 3 weeks after colorectal cancer surgery. In: Surgical Endoscopy and Other Interventional Techniques. 2011 ; Vol. 25, No. 6. pp. 1939-1944.
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