Plasma proline and leucine kinetics: Response to 4 wk with proline-free diets in young adults

Takeyuki Hiramatsu, Joaquin Cortiella, J. Sergio Marchini, Thomas E. Chapman, Vernon R. Young

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The effects of removing proline from the diet on plasma leucine and proline kinetics were investigated. After a 1-wk control period, during which young adult men received a diet containing a complete L-amino acid mixture, seven subjects were given for 4 wk a diet devoid of proline (group 1); six received a diet devoid of proline, arginine, aspartate, glutamate, and serine (group 2); and seven continued with the complete diet (group 3). At the end of the control and 4-wk periods subjects were given a continuous, (3-h fast, 5-h fed) intravenous infusion of L-[1-13C]leucine and L-[5.5-2H]proline. Plasma proline was reduced significantly, especially during the fed state, in groups 1 and 2 after the 4-wk diet periods. Small but statistically significant (P < 0.05) reductions occurred in nonoxidative leucine disappearance and leucine appearance during the fasted state in group 2. Proline fluxes decreased by ≃50% in fasted and fed states in groups 1 and 2. Mean de novo proline synthesis during the fasted state declined markedly (P < 0.05) after 4 wk in groups 1 and 2.

Original languageEnglish (US)
Pages (from-to)207-215
Number of pages9
JournalAmerican Journal of Clinical Nutrition
Issue number2
StatePublished - Aug 1994

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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