Plasmalemmal V-H+-ATPases regulate intracellular pH in human lung microvascular endothelial cells

Jose Rojas, Souad R. Sennoune, Debasish Maiti, Gloria M. Martínez, Karina Bakunts, Donald E. Wesson, Raul Martínez-Zaguilán

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The lung endothelium layer is exposed to continuous CO2 transit which exposes the endothelium to a substantial acid load that could be detrimental to cell function. The Na+/H+ exchanger and HCO3 --dependent H+-transporting mechanisms regulate intracellular pH (pHcyt) in most cells. Cells that cope with high acid loads might require additional primary energy-dependent mechanisms. V-H+-ATPases localized at the plasma membranes (pmV-ATPases) have emerged as a novel pH regulatory system. We hypothesized that human lung microvascular endothelial (HLMVE) cells use pmV-ATPases, in addition to Na +/H+ exchanger and HCO3 --based H+-transporting mechanisms, to maintain pHcyt homeostasis. Immunocytochemical studies revealed V-H+-ATPase at the plasma membrane, in addition to the predicted distribution in vacuolar compartments. Acid-loaded HLMVE cells exhibited proton fluxes in the absence of Na+ and HCO3 - that were similar to those observed in the presence of either Na+, or Na+ and HCO3 -. The Na+- and HCO3 --independent pHcyt recovery was inhibited by bafilomycin A1, a V-H +-ATPase inhibitor. These studies show a Na+- and HCO 3 --independent pHcyt regulatory mechanism in HLMVE cells that is mediated by pmV-ATPases.

Original languageEnglish (US)
Pages (from-to)1123-1132
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number4
DOIs
StatePublished - Aug 6 2004
Externally publishedYes

Fingerprint

Proton-Translocating ATPases
Endothelial cells
Adenosine Triphosphatases
Sodium-Hydrogen Antiporter
Endothelial Cells
Cell membranes
Lung
Acids
Endothelium
Cell Membrane
Protons
Fluxes
Recovery
Homeostasis

Keywords

  • Capillary endothelium
  • Fluorescence spectroscopy
  • Immunocytochemistry
  • Intracellular pH
  • Proton pumps

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rojas, J., Sennoune, S. R., Maiti, D., Martínez, G. M., Bakunts, K., Wesson, D. E., & Martínez-Zaguilán, R. (2004). Plasmalemmal V-H+-ATPases regulate intracellular pH in human lung microvascular endothelial cells. Biochemical and Biophysical Research Communications, 320(4), 1123-1132. https://doi.org/10.1016/j.bbrc.2004.06.068

Plasmalemmal V-H+-ATPases regulate intracellular pH in human lung microvascular endothelial cells. / Rojas, Jose; Sennoune, Souad R.; Maiti, Debasish; Martínez, Gloria M.; Bakunts, Karina; Wesson, Donald E.; Martínez-Zaguilán, Raul.

In: Biochemical and Biophysical Research Communications, Vol. 320, No. 4, 06.08.2004, p. 1123-1132.

Research output: Contribution to journalArticle

Rojas, J, Sennoune, SR, Maiti, D, Martínez, GM, Bakunts, K, Wesson, DE & Martínez-Zaguilán, R 2004, 'Plasmalemmal V-H+-ATPases regulate intracellular pH in human lung microvascular endothelial cells', Biochemical and Biophysical Research Communications, vol. 320, no. 4, pp. 1123-1132. https://doi.org/10.1016/j.bbrc.2004.06.068
Rojas, Jose ; Sennoune, Souad R. ; Maiti, Debasish ; Martínez, Gloria M. ; Bakunts, Karina ; Wesson, Donald E. ; Martínez-Zaguilán, Raul. / Plasmalemmal V-H+-ATPases regulate intracellular pH in human lung microvascular endothelial cells. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 320, No. 4. pp. 1123-1132.
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