Plasmalemmal vacuolar H+-ATPase is decreased in microvascular endothelial cells from a diabetic model

Jose Rojas, Souad R. Sennoune, Gloria M. Martinez, Karina Bakunts, Cynthia J. Meininger, Guoyao Wu, Donald E. Wesson, Elisabeth A. Seftor, Mary J C Hendrix, Raul Martínez-Zaguilán

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Angiogenesis requires invasion of extracellular matrix (ECM) proteins by endothelial cells and occurs in hypoxic and acidic environments that are not conducive for cell growth and survival. We hypothesize that angiogenic cells must exhibit a unique system to regulate their cytosolic pH in order to cope with these harsh conditions. The plasmalemmal vacuolar type H+-ATPase (pmV-ATPase) is used by cells exhibiting an invasive phenotype. Because angiogenesis is impaired in diabetes, we hypothesized that pmV-ATPase is decreased in microvascular endothelial cells from diabetic rats. The in vitro angiogenesis assays demonstrated that endothelial cells were unable to form capillary-like structures in diabetes. The proton fluxes were slower in cells from diabetic than normal model, regardless of the presence or absence of Na+ and HCO3 - and were suppressed by V-H +-ATPase inhibitors. Immunocytochemical data revealed that pmV-ATPases were inconspicuous at the plasma membrane of cells from diabetic whereas in normal cells were prominent. The pmV-ATPase activity was lower in cells from diabetic than normal models. Inhibition of V-H+-ATPase suppresses invasion/migration of normal cells, but have minor effects in cells from diabetic models. These novel observations suggest that the angiogenic abnormalities in diabetes involve a decrease in pmV-ATPase in microvascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)190-200
Number of pages11
JournalJournal of Cellular Physiology
Volume201
Issue number2
DOIs
StatePublished - Nov 2004
Externally publishedYes

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Vacuolar Proton-Translocating ATPases
Endothelial cells
Endothelial Cells
Medical problems
Proton-Translocating ATPases
Extracellular Matrix Proteins
Cell growth
Cell membranes
Adenosine Triphosphatases
Protons
Rats
Assays
Cells
Fluxes
Plasma Cells
Cell Movement
Cell Survival
Cell Membrane
Phenotype
Growth

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Rojas, J., Sennoune, S. R., Martinez, G. M., Bakunts, K., Meininger, C. J., Wu, G., ... Martínez-Zaguilán, R. (2004). Plasmalemmal vacuolar H+-ATPase is decreased in microvascular endothelial cells from a diabetic model. Journal of Cellular Physiology, 201(2), 190-200. https://doi.org/10.1002/jcp.20059

Plasmalemmal vacuolar H+-ATPase is decreased in microvascular endothelial cells from a diabetic model. / Rojas, Jose; Sennoune, Souad R.; Martinez, Gloria M.; Bakunts, Karina; Meininger, Cynthia J.; Wu, Guoyao; Wesson, Donald E.; Seftor, Elisabeth A.; Hendrix, Mary J C; Martínez-Zaguilán, Raul.

In: Journal of Cellular Physiology, Vol. 201, No. 2, 11.2004, p. 190-200.

Research output: Contribution to journalArticle

Rojas, J, Sennoune, SR, Martinez, GM, Bakunts, K, Meininger, CJ, Wu, G, Wesson, DE, Seftor, EA, Hendrix, MJC & Martínez-Zaguilán, R 2004, 'Plasmalemmal vacuolar H+-ATPase is decreased in microvascular endothelial cells from a diabetic model', Journal of Cellular Physiology, vol. 201, no. 2, pp. 190-200. https://doi.org/10.1002/jcp.20059
Rojas, Jose ; Sennoune, Souad R. ; Martinez, Gloria M. ; Bakunts, Karina ; Meininger, Cynthia J. ; Wu, Guoyao ; Wesson, Donald E. ; Seftor, Elisabeth A. ; Hendrix, Mary J C ; Martínez-Zaguilán, Raul. / Plasmalemmal vacuolar H+-ATPase is decreased in microvascular endothelial cells from a diabetic model. In: Journal of Cellular Physiology. 2004 ; Vol. 201, No. 2. pp. 190-200.
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