Plasmodium falciparum (Haemosporodia: Plasmodiidae) and O'nyong-nyong Virus Development in a Transgenic Anopheles gambiae (Diptera: Culicidae) Strain

John D. Mumford, Carole A. Long, Scott Weaver, Katzutoyo Miura, Eryu Wang, Rachel Rotenberry, Ellen M. Dotson, Mark Q. Benedict

Research output: Contribution to journalArticle

Abstract

Transgenic Anopheles gambiae Giles (Diptera: Culicidae) mosquitoes have been developed that confer sexual sterility on males that carry a transgene encoding a protein which cuts ribosomal DNA. A relevant risk concern with transgenic mosquitoes is that their capacity to transmit known pathogens could be greater than the unmodified form. In this study, the ability to develop two human pathogens in these transgenic mosquitoes carrying a homing endonuclease which is expressed in the testes was compared with its nontransgenic siblings. Infections were performed with Plasmodium falciparum (Welch) and o'nyong-nyong virus (ONNV) and the results between the transgenic and nontransgenic sibling females were compared. There was no difference observed with ONNV isolate SG650 in intrathoracic infections or the 50% oral infectious dose measured at 14 d postinfection or in mean body titers. Some significant differences were observed for leg titers at the medium and highest doses for those individuals in which virus titer could be detected. No consistent difference was observed between the transgenic and nontransgenic comparator females in their ability to develop P. falciparum NF54 strain parasites. This particular transgene caused no significant effect in the ability of mosquitoes to become infected by these two pathogens in this genetic background. These results are discussed in the context of risk to human health if these transgenic individuals were present in the environment.

Original languageEnglish (US)
Pages (from-to)936-941
Number of pages6
JournalJournal of medical entomology
Volume56
Issue number4
DOIs
StatePublished - Jun 27 2019

Fingerprint

O'nyong-nyong virus
O'nyong-nyong Virus
Anopheles gambiae
Plasmodium falciparum
Culicidae
Diptera
genetically modified organisms
Transgenes
transgenes
pathogens
Viruses
Endonucleases
Male Infertility
viruses
Ribosomal DNA
Infection
Viral Load
Testis
dosage
viral load

Keywords

  • arbovirus
  • competence
  • malaria
  • risk
  • transgenic

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Plasmodium falciparum (Haemosporodia : Plasmodiidae) and O'nyong-nyong Virus Development in a Transgenic Anopheles gambiae (Diptera: Culicidae) Strain. / Mumford, John D.; Long, Carole A.; Weaver, Scott; Miura, Katzutoyo; Wang, Eryu; Rotenberry, Rachel; Dotson, Ellen M.; Benedict, Mark Q.

In: Journal of medical entomology, Vol. 56, No. 4, 27.06.2019, p. 936-941.

Research output: Contribution to journalArticle

Mumford, John D. ; Long, Carole A. ; Weaver, Scott ; Miura, Katzutoyo ; Wang, Eryu ; Rotenberry, Rachel ; Dotson, Ellen M. ; Benedict, Mark Q. / Plasmodium falciparum (Haemosporodia : Plasmodiidae) and O'nyong-nyong Virus Development in a Transgenic Anopheles gambiae (Diptera: Culicidae) Strain. In: Journal of medical entomology. 2019 ; Vol. 56, No. 4. pp. 936-941.
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