Plasmodium ookinete-secreted proteins secreted through a common micronemal pathway are targets of blocking malaria transmission

Fengwu Li, Thomas J. Templeton, Vsevolod Popov, Jason Comer, Takafumi Tsuboi, Motomi Torii, Joseph M. Vinetz

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The mosquito midgut ookinete stage of the malaria parasite, Plasmodium, possesses microneme secretory organelles that mediate locomotion and midgut wall egress to establish sporogonic stages and subsequent transmission. The purpose of this study was 2-fold: 1) to determine whether there exists a single micronemal population with respect to soluble and membrane-associated secreted proteins; and 2) to evaluate the ookinete micronemal proteins chitinase (PgCHT1), circumsporozoite and TRAP-related protein (CTRP), and von Willebrand factor A domain-related protein (WARP) as immunological targets eliciting sera-blocking malaria parasite infectivity to mosquitoes. Indirect immunofluorescence localization studies in Plasmodium gallinaceum using specific antisera showed that all three proteins are distributed intracellularly with a similar granular cytoplasmic appearance and with focal concentration of PgCHT1 and PgCTRP, but not PgWARP, at the ookinete apical end. Immunogold double-labeling electron microscopy, using antisera against the membrane-associated protein CTRP and the soluble WARP, showed that these two proteins co-localized to the same micronemal population. Within the microneme CTRP was associated peripherally at the microneme membrane, whereas PgCHT1 and WARP were diffuse within the micronemal lumen. Sera produced against Plasmodium falciparum WARP significantly reduced the infectivity of P. gallinaceum to Aedes aegypti and P. falciparum to Anopheles mosquitoes. Antisera against PgCTRP and PgCHT1 also significantly reduced the infectivity of P. gallinaceum for A. aegypti. These results support the concept that ookinete micronemal proteins may constitute a general class of malaria transmission-blocking vaccine candidates.

Original languageEnglish (US)
Pages (from-to)26635-26644
Number of pages10
JournalJournal of Biological Chemistry
Volume279
Issue number25
DOIs
StatePublished - Jun 18 2004

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Plasmodium
Malaria
Plasmodium gallinaceum
Culicidae
Proteins
Immune Sera
Plasmodium falciparum
Membrane Proteins
Parasites
Plasmodium malariae
Chitinases
Anopheles
Aedes
von Willebrand Factor
Locomotion
Indirect Fluorescent Antibody Technique
Serum
Organelles
Population
Membranes

ASJC Scopus subject areas

  • Biochemistry

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Plasmodium ookinete-secreted proteins secreted through a common micronemal pathway are targets of blocking malaria transmission. / Li, Fengwu; Templeton, Thomas J.; Popov, Vsevolod; Comer, Jason; Tsuboi, Takafumi; Torii, Motomi; Vinetz, Joseph M.

In: Journal of Biological Chemistry, Vol. 279, No. 25, 18.06.2004, p. 26635-26644.

Research output: Contribution to journalArticle

Li, Fengwu ; Templeton, Thomas J. ; Popov, Vsevolod ; Comer, Jason ; Tsuboi, Takafumi ; Torii, Motomi ; Vinetz, Joseph M. / Plasmodium ookinete-secreted proteins secreted through a common micronemal pathway are targets of blocking malaria transmission. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 25. pp. 26635-26644.
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