Plasticity in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits in the rat dorsal horn following deafferentation

Susan M. Carlton, Gregory L. Hargett, Richard E. Coggeshall

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Immunostaining for GluR1 and GluR2/3 subunits of the α-amino-3-hydroxy- 5-methyl-4-isoxazolepropionic acid (AMPA) receptor is prominent in laminae I and II of the normal dorsal horn, with much less staining in deeper laminae. Three days after dorsal rhizotomy, image analysis demonstrates a significant decrease in staining density for both antibodies in the superficial dorsal horn. By contrast, staining density is significantly increased in laminae III, IV and the reticulated region of lamina V for GluR1 only. This increase suggests that AMPA receptors contribute to deafferentation or radicular pain by at least two mechanisms: (1) up-regulation of GluR1 subunits of the AMPA receptor in deeper laminae, and (2) enhanced Ca2+ permeability of dorsal horn neurons because AMPA receptors lacking the GluR2 subunit are Ca2+ permeable. Glutamate activation of these receptors could initiate or potentiate second messenger cascades that could well contribute to neuronal changes documented in the dorsal horn of deafferented animals.

    Original languageEnglish (US)
    Pages (from-to)21-24
    Number of pages4
    JournalNeuroscience Letters
    Volume242
    Issue number1
    DOIs
    StatePublished - Feb 6 1998

    Fingerprint

    Acids
    Substantia Gelatinosa
    AMPA Receptors
    Staining and Labeling
    Rhizotomy
    Posterior Horn Cells
    Glutamate Receptors
    Second Messenger Systems
    Permeability
    Up-Regulation
    Spinal Cord Dorsal Horn
    Pain
    Antibodies

    Keywords

    • Deafferentation
    • GluR1
    • GluR2/3
    • Primary afferent

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Plasticity in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits in the rat dorsal horn following deafferentation. / Carlton, Susan M.; Hargett, Gregory L.; Coggeshall, Richard E.

    In: Neuroscience Letters, Vol. 242, No. 1, 06.02.1998, p. 21-24.

    Research output: Contribution to journalArticle

    Carlton, Susan M. ; Hargett, Gregory L. ; Coggeshall, Richard E. / Plasticity in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits in the rat dorsal horn following deafferentation. In: Neuroscience Letters. 1998 ; Vol. 242, No. 1. pp. 21-24.
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