Plasticity in intact Aδ- and C-fibers contributes to cold hypersensitivity in neuropathic rats

G. Ji, S. Zhou, M. Y. Kochukov, K. N. Westlund, S. M. Carlton

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Cold hypersensitivity is a common sensory abnormality accompanying peripheral neuropathies and is difficult to treat. Progress has been made in understanding peripheral mechanisms underlying neuropathic pain but little is known concerning peripheral mechanisms of cold hypersensitivity. The aim of this study was to analyze the contribution of uninjured primary afferents to the cold hypersensitivity that develops in neuropathic rats. Rats with a lumbar 5 (L5) and L6 spinal nerve ligation (SNL, Chung model) but not sham, developed mechanical allodynia, evidenced by decreased paw withdrawal thresholds and increased magnitude of response to von Frey stimulation. Cold hypersensitivity also developed in SNL but not sham rats, evidenced by enhanced nociceptive behaviors induced by placement on a cold plate (6 °C) or application of icilin (a transient receptor potential M8 (TRPM8)/transient receptor potential A1 (TRPA1) receptor agonist) to nerve-injured hind paws. Single fiber recordings demonstrated that the mean conduction velocities of intact L4 cutaneous Aδ- and C-fibers were not different between naive and SNL rats; however, mechanical thresholds of the Aδ- but not the C-fibers were significantly decreased in SNL compared with naive. There was a higher prevalence of C-mechanoheat-cold (CMHC) fibers in SNL compared with naive, but the overall percentage of cold-sensitive C-fibers was not significantly increased compared with naive. This was in contrast to the numerous changes in Aδ-fibers: the percentage of L4 cold sensitive Aδ-, but not C-fibers, was significantly increased, the percentage of L4 icilin-sensitive Aδ-, but not C-fibers, was significantly increased, the icilin-induced activity of L4 Aδ-, but not C-fibers, was significantly increased. Icilin-induced activity was blocked by the TRPA1 antagonist Ruthenium Red. The results indicate plasticity in both Aδ- and C-uninjured fibers, but Aδ fibers appear to provide a major contribution to cold hypersensitivity in neuropathic rats.

    Original languageEnglish (US)
    Pages (from-to)182-193
    Number of pages12
    JournalNeuroscience
    Volume150
    Issue number1
    DOIs
    StatePublished - Nov 30 2007

    Fingerprint

    Unmyelinated Nerve Fibers
    Myelinated Nerve Fibers
    Spinal Nerves
    Ruthenium
    Hyperalgesia
    Neuralgia
    Peripheral Nervous System Diseases
    Ligation
    Cold Hypersensitivity
    Skin
    icilin

    Keywords

    • cold allodynia
    • neuropathic pain
    • peripheral neuropathy
    • sensitization
    • TRPA1
    • TRPM8

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Plasticity in intact Aδ- and C-fibers contributes to cold hypersensitivity in neuropathic rats. / Ji, G.; Zhou, S.; Kochukov, M. Y.; Westlund, K. N.; Carlton, S. M.

    In: Neuroscience, Vol. 150, No. 1, 30.11.2007, p. 182-193.

    Research output: Contribution to journalArticle

    Ji, G. ; Zhou, S. ; Kochukov, M. Y. ; Westlund, K. N. ; Carlton, S. M. / Plasticity in intact Aδ- and C-fibers contributes to cold hypersensitivity in neuropathic rats. In: Neuroscience. 2007 ; Vol. 150, No. 1. pp. 182-193.
    @article{8fc5f89e0de844d0baa0ffa84bffaba1,
    title = "Plasticity in intact Aδ- and C-fibers contributes to cold hypersensitivity in neuropathic rats",
    abstract = "Cold hypersensitivity is a common sensory abnormality accompanying peripheral neuropathies and is difficult to treat. Progress has been made in understanding peripheral mechanisms underlying neuropathic pain but little is known concerning peripheral mechanisms of cold hypersensitivity. The aim of this study was to analyze the contribution of uninjured primary afferents to the cold hypersensitivity that develops in neuropathic rats. Rats with a lumbar 5 (L5) and L6 spinal nerve ligation (SNL, Chung model) but not sham, developed mechanical allodynia, evidenced by decreased paw withdrawal thresholds and increased magnitude of response to von Frey stimulation. Cold hypersensitivity also developed in SNL but not sham rats, evidenced by enhanced nociceptive behaviors induced by placement on a cold plate (6 °C) or application of icilin (a transient receptor potential M8 (TRPM8)/transient receptor potential A1 (TRPA1) receptor agonist) to nerve-injured hind paws. Single fiber recordings demonstrated that the mean conduction velocities of intact L4 cutaneous Aδ- and C-fibers were not different between naive and SNL rats; however, mechanical thresholds of the Aδ- but not the C-fibers were significantly decreased in SNL compared with naive. There was a higher prevalence of C-mechanoheat-cold (CMHC) fibers in SNL compared with naive, but the overall percentage of cold-sensitive C-fibers was not significantly increased compared with naive. This was in contrast to the numerous changes in Aδ-fibers: the percentage of L4 cold sensitive Aδ-, but not C-fibers, was significantly increased, the percentage of L4 icilin-sensitive Aδ-, but not C-fibers, was significantly increased, the icilin-induced activity of L4 Aδ-, but not C-fibers, was significantly increased. Icilin-induced activity was blocked by the TRPA1 antagonist Ruthenium Red. The results indicate plasticity in both Aδ- and C-uninjured fibers, but Aδ fibers appear to provide a major contribution to cold hypersensitivity in neuropathic rats.",
    keywords = "cold allodynia, neuropathic pain, peripheral neuropathy, sensitization, TRPA1, TRPM8",
    author = "G. Ji and S. Zhou and Kochukov, {M. Y.} and Westlund, {K. N.} and Carlton, {S. M.}",
    year = "2007",
    month = "11",
    day = "30",
    doi = "10.1016/j.neuroscience.2007.09.002",
    language = "English (US)",
    volume = "150",
    pages = "182--193",
    journal = "Neuroscience",
    issn = "0306-4522",
    publisher = "Elsevier Limited",
    number = "1",

    }

    TY - JOUR

    T1 - Plasticity in intact Aδ- and C-fibers contributes to cold hypersensitivity in neuropathic rats

    AU - Ji, G.

    AU - Zhou, S.

    AU - Kochukov, M. Y.

    AU - Westlund, K. N.

    AU - Carlton, S. M.

    PY - 2007/11/30

    Y1 - 2007/11/30

    N2 - Cold hypersensitivity is a common sensory abnormality accompanying peripheral neuropathies and is difficult to treat. Progress has been made in understanding peripheral mechanisms underlying neuropathic pain but little is known concerning peripheral mechanisms of cold hypersensitivity. The aim of this study was to analyze the contribution of uninjured primary afferents to the cold hypersensitivity that develops in neuropathic rats. Rats with a lumbar 5 (L5) and L6 spinal nerve ligation (SNL, Chung model) but not sham, developed mechanical allodynia, evidenced by decreased paw withdrawal thresholds and increased magnitude of response to von Frey stimulation. Cold hypersensitivity also developed in SNL but not sham rats, evidenced by enhanced nociceptive behaviors induced by placement on a cold plate (6 °C) or application of icilin (a transient receptor potential M8 (TRPM8)/transient receptor potential A1 (TRPA1) receptor agonist) to nerve-injured hind paws. Single fiber recordings demonstrated that the mean conduction velocities of intact L4 cutaneous Aδ- and C-fibers were not different between naive and SNL rats; however, mechanical thresholds of the Aδ- but not the C-fibers were significantly decreased in SNL compared with naive. There was a higher prevalence of C-mechanoheat-cold (CMHC) fibers in SNL compared with naive, but the overall percentage of cold-sensitive C-fibers was not significantly increased compared with naive. This was in contrast to the numerous changes in Aδ-fibers: the percentage of L4 cold sensitive Aδ-, but not C-fibers, was significantly increased, the percentage of L4 icilin-sensitive Aδ-, but not C-fibers, was significantly increased, the icilin-induced activity of L4 Aδ-, but not C-fibers, was significantly increased. Icilin-induced activity was blocked by the TRPA1 antagonist Ruthenium Red. The results indicate plasticity in both Aδ- and C-uninjured fibers, but Aδ fibers appear to provide a major contribution to cold hypersensitivity in neuropathic rats.

    AB - Cold hypersensitivity is a common sensory abnormality accompanying peripheral neuropathies and is difficult to treat. Progress has been made in understanding peripheral mechanisms underlying neuropathic pain but little is known concerning peripheral mechanisms of cold hypersensitivity. The aim of this study was to analyze the contribution of uninjured primary afferents to the cold hypersensitivity that develops in neuropathic rats. Rats with a lumbar 5 (L5) and L6 spinal nerve ligation (SNL, Chung model) but not sham, developed mechanical allodynia, evidenced by decreased paw withdrawal thresholds and increased magnitude of response to von Frey stimulation. Cold hypersensitivity also developed in SNL but not sham rats, evidenced by enhanced nociceptive behaviors induced by placement on a cold plate (6 °C) or application of icilin (a transient receptor potential M8 (TRPM8)/transient receptor potential A1 (TRPA1) receptor agonist) to nerve-injured hind paws. Single fiber recordings demonstrated that the mean conduction velocities of intact L4 cutaneous Aδ- and C-fibers were not different between naive and SNL rats; however, mechanical thresholds of the Aδ- but not the C-fibers were significantly decreased in SNL compared with naive. There was a higher prevalence of C-mechanoheat-cold (CMHC) fibers in SNL compared with naive, but the overall percentage of cold-sensitive C-fibers was not significantly increased compared with naive. This was in contrast to the numerous changes in Aδ-fibers: the percentage of L4 cold sensitive Aδ-, but not C-fibers, was significantly increased, the percentage of L4 icilin-sensitive Aδ-, but not C-fibers, was significantly increased, the icilin-induced activity of L4 Aδ-, but not C-fibers, was significantly increased. Icilin-induced activity was blocked by the TRPA1 antagonist Ruthenium Red. The results indicate plasticity in both Aδ- and C-uninjured fibers, but Aδ fibers appear to provide a major contribution to cold hypersensitivity in neuropathic rats.

    KW - cold allodynia

    KW - neuropathic pain

    KW - peripheral neuropathy

    KW - sensitization

    KW - TRPA1

    KW - TRPM8

    UR - http://www.scopus.com/inward/record.url?scp=36649014923&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=36649014923&partnerID=8YFLogxK

    U2 - 10.1016/j.neuroscience.2007.09.002

    DO - 10.1016/j.neuroscience.2007.09.002

    M3 - Article

    C2 - 17945425

    AN - SCOPUS:36649014923

    VL - 150

    SP - 182

    EP - 193

    JO - Neuroscience

    JF - Neuroscience

    SN - 0306-4522

    IS - 1

    ER -